PDF(Pubmed)
Abstract：
OBJECTIVE: Periodontitis is a multifactorial disease that affects a wide range of populations. However, its pathogenesis remains unclear. miRNAs are now considered potential diagnostic markers for many inflammatory diseases. Thus, the aim of this study was to assess the expression of microRNA-223(miRNA-223) and microRNA-214 (miRNA-214) in gingival crevicular fluid (GCF) of smoker and nonsmoker with periodontitis.
METHODS: We conducted a prospective study among 42 participants: 14 healthy controls, 14 nonsmoker periodontitis participants, and 14 smokers with periodontitis. Eligibility criteria for inclusion were consecutive adults, aged 20-60 years, with stage III periodontitis grade B/C and no systemic diseases. All consenting participants had gingival crevicular fluid samples collected after diagnosis to assess miRNA-214 and -223 by quantitative real-time polymerase chain reaction assay.
RESULTS: ROC curve analyses for the non-smoker periodontitis group showed that miR-214 as a predictor in comparison to miR-223 had higher sensitivity [92.86%-64.29%], same specificity [100%], and a significantly higher area under the curve [0.974-0.796] respectively (p = 0.036). As for the smoker periodontitis group, a ROC curve with miR-214 as predictor in comparison to miR-223 had higher sensitivity [100%-71.43%], same specificity [100%], and a non-significantly higher area under the curve [1-0.872], respectively (p = 0.059).
CONCLUSIONS: Both miRNA-214 and 223 are reliable potential diagnostic markers for periodontitis, with miRNA-214 being more accurate for smokers with periodontitis.
CONCLUSIONS: Both miRNA-214 and 223 could be considered for potential chair-side diagnostics, by simply collecting GCF detecting the disease in its first steps and aid in preventing unrepairable damage.
METHODS: We conducted a prospective study among 42 participants: 14 healthy controls, 14 nonsmoker periodontitis participants, and 14 smokers with periodontitis. Eligibility criteria for inclusion were consecutive adults, aged 20-60 years, with stage III periodontitis grade B/C and no systemic diseases. All consenting participants had gingival crevicular fluid samples collected after diagnosis to assess miRNA-214 and -223 by quantitative real-time polymerase chain reaction assay.
RESULTS: ROC curve analyses for the non-smoker periodontitis group showed that miR-214 as a predictor in comparison to miR-223 had higher sensitivity [92.86%-64.29%], same specificity [100%], and a significantly higher area under the curve [0.974-0.796] respectively (p = 0.036). As for the smoker periodontitis group, a ROC curve with miR-214 as predictor in comparison to miR-223 had higher sensitivity [100%-71.43%], same specificity [100%], and a non-significantly higher area under the curve [1-0.872], respectively (p = 0.059).
CONCLUSIONS: Both miRNA-214 and 223 are reliable potential diagnostic markers for periodontitis, with miRNA-214 being more accurate for smokers with periodontitis.
CONCLUSIONS: Both miRNA-214 and 223 could be considered for potential chair-side diagnostics, by simply collecting GCF detecting the disease in its first steps and aid in preventing unrepairable damage.
摘要：
目的：牙周炎是一种影响广泛人群的多因素疾病。然而,其发病机制尚不清楚。miRNA现在被认为是许多炎性疾病的潜在诊断标志物。因此，本研究的目的是评估患有牙周炎的吸烟者和非吸烟者龈沟液(GCF)中microRNA-223(miRNA-223)和microRNA-214(miRNA-214)的表达.
方法：我们在42名参与者中进行了一项前瞻性研究：14名健康对照，14名非吸烟者牙周炎参与者，和14名患有牙周炎的吸烟者。入选标准是连续的成年人，20-60岁,Ⅲ期牙周炎B/C级，无全身性疾病。所有同意的参与者在诊断后收集牙龈沟液样品，以通过定量实时聚合酶链反应测定评估miRNA-214和-223。
结果：非吸烟者牙周炎组的ROC曲线分析显示，与miR-223相比，miR-214作为预测因子具有更高的敏感性[92.86%-64.29%]。相同的特异性[100%]，曲线下面积显著增加[0.974-0.796](p=0.036)。至于吸烟者牙周炎组，与miR-223相比，以miR-214为预测因子的ROC曲线具有更高的灵敏度[100％-71.43％]，相同的特异性[100%]，和非显著较高的曲线下面积[1-0.872]，分别(p=0.059)。
结论：miRNA-214和223都是可靠的牙周炎潜在诊断标记，miRNA-214对患有牙周炎的吸烟者更准确。
结论：miRNA-214和223都可以被考虑用于潜在的椅子侧诊断，通过简单地收集GCF检测疾病的第一步，并帮助防止不可修复的损害。
方法：我们在42名参与者中进行了一项前瞻性研究：14名健康对照，14名非吸烟者牙周炎参与者，和14名患有牙周炎的吸烟者。入选标准是连续的成年人，20-60岁,Ⅲ期牙周炎B/C级，无全身性疾病。所有同意的参与者在诊断后收集牙龈沟液样品，以通过定量实时聚合酶链反应测定评估miRNA-214和-223。
结果：非吸烟者牙周炎组的ROC曲线分析显示，与miR-223相比，miR-214作为预测因子具有更高的敏感性[92.86%-64.29%]。相同的特异性[100%]，曲线下面积显著增加[0.974-0.796](p=0.036)。至于吸烟者牙周炎组，与miR-223相比，以miR-214为预测因子的ROC曲线具有更高的灵敏度[100％-71.43％]，相同的特异性[100%]，和非显著较高的曲线下面积[1-0.872]，分别(p=0.059)。
结论：miRNA-214和223都是可靠的牙周炎潜在诊断标记，miRNA-214对患有牙周炎的吸烟者更准确。
结论：miRNA-214和223都可以被考虑用于潜在的椅子侧诊断，通过简单地收集GCF检测疾病的第一步，并帮助防止不可修复的损害。
