METHODS: We conducted a prospective study among 42 participants: 14 healthy controls, 14 nonsmoker periodontitis participants, and 14 smokers with periodontitis. Eligibility criteria for inclusion were consecutive adults, aged 20-60 years, with stage III periodontitis grade B/C and no systemic diseases. All consenting participants had gingival crevicular fluid samples collected after diagnosis to assess miRNA-214 and -223 by quantitative real-time polymerase chain reaction assay.
RESULTS: ROC curve analyses for the non-smoker periodontitis group showed that miR-214 as a predictor in comparison to miR-223 had higher sensitivity [92.86%-64.29%], same specificity [100%], and a significantly higher area under the curve [0.974-0.796] respectively (p = 0.036). As for the smoker periodontitis group, a ROC curve with miR-214 as predictor in comparison to miR-223 had higher sensitivity [100%-71.43%], same specificity [100%], and a non-significantly higher area under the curve [1-0.872], respectively (p = 0.059).
CONCLUSIONS: Both miRNA-214 and 223 are reliable potential diagnostic markers for periodontitis, with miRNA-214 being more accurate for smokers with periodontitis.
CONCLUSIONS: Both miRNA-214 and 223 could be considered for potential chair-side diagnostics, by simply collecting GCF detecting the disease in its first steps and aid in preventing unrepairable damage.
方法:我们在42名参与者中进行了一项前瞻性研究:14名健康对照,14名非吸烟者牙周炎参与者,和14名患有牙周炎的吸烟者。入选标准是连续的成年人,20-60岁,Ⅲ期牙周炎B/C级,无全身性疾病。所有同意的参与者在诊断后收集牙龈沟液样品,以通过定量实时聚合酶链反应测定评估miRNA-214和-223。
结果:非吸烟者牙周炎组的ROC曲线分析显示,与miR-223相比,miR-214作为预测因子具有更高的敏感性[92.86%-64.29%]。相同的特异性[100%],曲线下面积显著增加[0.974-0.796](p=0.036)。至于吸烟者牙周炎组,与miR-223相比,以miR-214为预测因子的ROC曲线具有更高的灵敏度[100%-71.43%],相同的特异性[100%],和非显著较高的曲线下面积[1-0.872],分别(p=0.059)。
结论:miRNA-214和223都是可靠的牙周炎潜在诊断标记,miRNA-214对患有牙周炎的吸烟者更准确。
结论:miRNA-214和223都可以被考虑用于潜在的椅子侧诊断,通过简单地收集GCF检测疾病的第一步,并帮助防止不可修复的损害。