Abstract：
Telomere-led rapid chromosome movements (RPMs) are a conserved characteristic of chromosome dynamics in meiosis. RPMs have been suggested to influence critical meiotic functions such as DNA repair and the association of the homologous chromosomes. Here, we describe a method using 3D time-lapse fluorescence imaging to monitor RPMs in Hoechst-stained mouse seminiferous tubules explants. We supplement visualization with customized quantitative motion analysis and in silico simulation. The ability to carry out live imaging, combined with quantitative image analysis, offers a sensitive tool to investigate the regulation of RPMs, chromosome reorganizations that precede dynamic mid-prophase events, and their contribution to faithful transmission of genetic information.
摘要：
端粒导致的快速染色体运动（RPM）是减数分裂中染色体动力学的保守特征。已经提出RPM影响关键减数分裂功能,例如DNA修复和同源染色体的关联。这里,我们描述了一种使用3D延时荧光成像来监测Hoechst染色的小鼠生精小管外植体的RPM的方法。我们通过定制的定量运动分析和计算机模拟来补充可视化。能够进行实时成像，结合定量图像分析，提供了一个敏感的工具来调查RPM的监管，在动态中期前期事件之前的染色体重组，以及它们对基因信息忠实传递的贡献。
