%0 Journal Article
%T Visualization and Quantification of Rapid Chromosome Movements at Early Stages of Mouse Meiosis.
%A de Almeida LP
%A Lee CY
%A Carbajal A
%A de Castro RO
%A Pezza RJ
%J Methods Mol Biol
%V 2818
%N 0
%D 2024
%M 39126474
暂无%R 10.1007/978-1-0716-3906-1_11
%X Telomere-led rapid chromosome movements (RPMs) are a conserved characteristic of chromosome dynamics in meiosis. RPMs have been suggested to influence critical meiotic functions such as DNA repair and the association of the homologous chromosomes. Here, we describe a method using 3D time-lapse fluorescence imaging to monitor RPMs in Hoechst-stained mouse seminiferous tubules explants. We supplement visualization with customized quantitative motion analysis and in silico simulation. The ability to carry out live imaging, combined with quantitative image analysis, offers a sensitive tool to investigate the regulation of RPMs, chromosome reorganizations that precede dynamic mid-prophase events, and their contribution to faithful transmission of genetic information.