Abstract：
BACKGROUND: Walking catfish, Clarias batrachus is one of the native and most popular freshwater catfish species in Indonesia. However, cultivation faces challenges, particularly due to the scarcity of larvae resulting from underdeveloped breeding technologies. Cryopreservation is a method of storing sperm to maintain viability for a long period and support the breeding technology of the fish. Cryoprotectant, in this context, plays an important role in determining the success of sperm cryopreservation.
OBJECTIVE: To determine the best type and concentration of cryoprotectant for cryopreservation of walking catfish sperm.
METHODS: A total of five different types of cryoprotectants, namely DMSO, glycerol, ethyl glycol, ethanol, and methanol, were tested at four concentration levels namely 0%, 5%, 10%, 15%, and 20%, each with four replications.
RESULTS: The type and concentration of cryoprotectant had a significant effect on sperm motility and viability (P < 0.05). The best outcomes were obtained with 5% DMSO and ethyl glycol, 10% glycerol and methanol, as well as 15% ethanol.
CONCLUSIONS: The highest motility and viability values were obtained with 5% DMSO, resulting in its recommendation for cryopreservation of walking catfish sperm. Doi.org/10.54680/fr24510110612.
OBJECTIVE: To determine the best type and concentration of cryoprotectant for cryopreservation of walking catfish sperm.
METHODS: A total of five different types of cryoprotectants, namely DMSO, glycerol, ethyl glycol, ethanol, and methanol, were tested at four concentration levels namely 0%, 5%, 10%, 15%, and 20%, each with four replications.
RESULTS: The type and concentration of cryoprotectant had a significant effect on sperm motility and viability (P < 0.05). The best outcomes were obtained with 5% DMSO and ethyl glycol, 10% glycerol and methanol, as well as 15% ethanol.
CONCLUSIONS: The highest motility and viability values were obtained with 5% DMSO, resulting in its recommendation for cryopreservation of walking catfish sperm. Doi.org/10.54680/fr24510110612.
摘要：
背景：走路的鲶鱼，Clariasbatrachus是印度尼西亚本地和最受欢迎的淡水cat鱼之一。然而,种植面临挑战，特别是由于繁殖技术不发达导致的幼虫稀缺。冷冻保存是一种储存精子以长期维持生存能力并支持鱼类繁殖技术的方法。冷冻保护剂,在这种情况下，对精子冷冻保存的成功与否起着重要的作用。
目的：确定行走鲶鱼精子冷冻保存的最佳冷冻保护剂种类和浓度。
方法：总共五种不同类型的冷冻保护剂，即DMSO，甘油，乙二醇，乙醇,和甲醇，在四个浓度水平下测试，即0%，5%,10%,15%,20%,每个都有四个重复。
结果：冷冻保护剂的种类和浓度对精子活力和活力有显著影响(P<0.05)。用5%DMSO和乙二醇获得最佳结果，10%甘油和甲醇，以及15%的乙醇。
结论：用5%DMSO获得了最高的运动和活力值，因此，它建议对行走的cat鱼精子进行冷冻保存。Doi.org/10.54680/fr24510110612。
目的：确定行走鲶鱼精子冷冻保存的最佳冷冻保护剂种类和浓度。
方法：总共五种不同类型的冷冻保护剂，即DMSO，甘油，乙二醇，乙醇,和甲醇，在四个浓度水平下测试，即0%，5%,10%,15%,20%,每个都有四个重复。
结果：冷冻保护剂的种类和浓度对精子活力和活力有显著影响(P<0.05)。用5%DMSO和乙二醇获得最佳结果，10%甘油和甲醇，以及15%的乙醇。
结论：用5%DMSO获得了最高的运动和活力值，因此，它建议对行走的cat鱼精子进行冷冻保存。Doi.org/10.54680/fr24510110612。
