OBJECTIVE: To determine the best type and concentration of cryoprotectant for cryopreservation of walking catfish sperm.
METHODS: A total of five different types of cryoprotectants, namely DMSO, glycerol, ethyl glycol, ethanol, and methanol, were tested at four concentration levels namely 0%, 5%, 10%, 15%, and 20%, each with four replications.
RESULTS: The type and concentration of cryoprotectant had a significant effect on sperm motility and viability (P < 0.05). The best outcomes were obtained with 5% DMSO and ethyl glycol, 10% glycerol and methanol, as well as 15% ethanol.
CONCLUSIONS: The highest motility and viability values were obtained with 5% DMSO, resulting in its recommendation for cryopreservation of walking catfish sperm. Doi.org/10.54680/fr24510110612.
目的:确定行走鲶鱼精子冷冻保存的最佳冷冻保护剂种类和浓度。
方法:总共五种不同类型的冷冻保护剂,即DMSO,甘油,乙二醇,乙醇,和甲醇,在四个浓度水平下测试,即0%,5%,10%,15%,20%,每个都有四个重复。
结果:冷冻保护剂的种类和浓度对精子活力和活力有显著影响(P<0.05)。用5%DMSO和乙二醇获得最佳结果,10%甘油和甲醇,以及15%的乙醇。
结论:用5%DMSO获得了最高的运动和活力值,因此,它建议对行走的cat鱼精子进行冷冻保存。Doi.org/10.54680/fr24510110612。