PDF(Pubmed)
Abstract：
The negative environmental and social impacts of food waste accumulation can be mitigated by utilizing bio-refineries\' approach where food waste is revalorized into high-value products, such as prodigiosin (PG), using microbial bioprocesses. The diverse biological activities of PG position it as a promising compound, but its high production cost and promiscuous bioactivity hinder its wide application. Metal ions can modulate the electronic properties of organic molecules, leading to novel mechanisms of action and increased target potency, while metal complex formation can improve the stability, solubility and bioavailability of the parent compound. The objectives of this study were optimizing PG production through bacterial fermentation using food waste, allowing good quantities of the pure natural product for further synthesizing and evaluating copper(II) and zinc(II) complexes with it. Their antimicrobial and anticancer activities were assessed, and their binding affinity toward biologically important molecules, bovine serum albumin (BSA) and DNA was investigated by fluorescence emission spectroscopy and molecular docking. The yield of 83.1 mg/L of pure PG was obtained when processed meat waste at 18 g/L was utilized as the sole fermentation substrate. The obtained complexes CuPG and ZnPG showed high binding affinity towards target site III of BSA, and molecular docking simulations highlighted the affinity of the compounds for DNA minor grooves.
摘要：
食物垃圾积累的负面环境和社会影响可以通过利用生物炼油厂的方法来减轻，在这种方法中，食物垃圾被重新价值为高价值产品。如prodigiosin(PG)，使用微生物生物过程。PG的多种生物活性使其成为有前途的化合物，但其高生产成本和混杂的生物活性阻碍了其广泛应用。金属离子可以调节有机分子的电子性质，导致新的作用机制和增加的目标效力，虽然金属络合物的形成可以提高稳定性，母体化合物的溶解度和生物利用度。这项研究的目的是通过使用食物垃圾进行细菌发酵来优化PG的生产，允许大量的纯天然产物用于进一步合成和评估与它的铜（II）和锌（II）络合物。评估了它们的抗菌和抗癌活性，以及它们对生物学重要分子的结合亲和力，通过荧光发射光谱和分子对接研究牛血清白蛋白(BSA)和DNA。当使用18g/L的加工肉类废物作为唯一的发酵底物时，获得了83.1mg/L的纯PG的产量。获得的复合物CuPG和ZnPG对BSA的靶位点III显示出高结合亲和力，和分子对接模拟强调了化合物对DNA小沟的亲和力。
