PDF(Pubmed)
Abstract：
Multiple drug resistance (MDR) has gained pronounced attention among Enterobacterales. The transfer of multiple antimicrobial resistance genes, frequently carried on conjugative incompatibility F (IncF) plasmids and facilitating interspecies resistance transmission, has been linked to Salmonella spp. and E. coli in broilers. In Egypt, the growing resistance is exacerbated by the limited clinical efficacy of many antimicrobials. In this study, IncF groups were screened and characterized in drug-resistant Salmonella spp. and E. coli isolated from broilers. The antimicrobial resistance profile, PCR-based replicon typing of bacterial isolates pre- and post-plasmid curing, and IncF replicon allele sequence typing were investigated. Five isolates of E. coli (5/31; 16.13%) and Salmonella spp. (5/36; 13.89%) were pan-susceptible to the examined antimicrobial agents, and 85.07% of tested isolates were MDR and extensively drug-resistant (XDR). Twelve MDR and XDR E. coli and Salmonella spp. isolates were examined for the existence of IncF replicons (FII, FIA, and FIB). They shared resistance to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanate, doxycycline, cefotaxime, and colistin. All isolates carried from one to two IncF replicons. The FII-FIA-FIB+ and FII-FIA+FIB- were the predominant replicon patterns. FIB was the most frequently detected replicon after plasmid curing. Three XDR E. coli isolates that were resistant to 12-14 antimicrobials carried a newly FIB replicon allele with four nucleotide substitutions: C99→A, G112→T, C113→T, and G114→A. These findings suggest that broilers are a significant reservoir of IncF replicons with highly divergent IncF-FIB plasmid incompatibility groups circulating among XDR Enterobacterales. Supporting these data with additional comprehensive epidemiological studies involving replicons other than the IncF can provide insights for implementing efficient policies to prevent the spreading of new replicons to humans.
摘要：
多重耐药性（MDR）已在肠杆菌中引起了广泛关注。多个抗菌素耐药基因的转移，经常进行共轭不相容性F（IncF）质粒，并促进种间抗性传播，与沙门氏菌有关.和大肠杆菌在肉鸡。在埃及,许多抗菌药物的临床疗效有限，加剧了耐药性的增长。在这项研究中,筛选IncF组，并对耐药沙门氏菌进行鉴定。和分离自肉仔鸡的大肠杆菌。抗菌素耐药性概况，基于PCR的质粒固化前后细菌分离株的复制子分型，和IncF复制子等位基因分型进行了研究。5株大肠杆菌(5/31;16.13%)和沙门氏菌。(5/36；13.89%)对所检查的抗菌剂普遍敏感，85.07%的检测菌株为MDR和广泛耐药(XDR)。12个MDR和XDR大肠杆菌和沙门氏菌。检查分离株是否存在IncF复制子(FII，国际汽联,和FIB）。他们对氨苄青霉素有共同的抗药性,氨苄西林/舒巴坦,阿莫西林/克拉维酸，多西环素,头孢噻肟,还有粘菌素.所有分离株携带一至两个IncF复制子。FII-FIA-FIB+和FII-FIA+FIB-是主要的复制子模式。FIB是质粒固化后最常见的复制子。三个对12-14种抗生素具有抗性的XDR大肠杆菌分离株携带一个新的FIB复制子等位基因，具有四个核苷酸取代：C99→A，G112→T,C113→T,和G114→A.这些发现表明，肉鸡是IncF复制子的重要储库，在XDR肠杆菌中循环的IncF-FIB质粒不相容组高度分散。通过涉及IncF以外的复制子的其他全面流行病学研究来支持这些数据，可以为实施有效的政策以防止新复制子向人类传播提供见解。
