PDF(Pubmed)
Abstract：
A T-cell-independent (TI) pathway activated by microbiota results in the generation of low-affinity homeostatic IgA with a critical role in intestinal homeostasis. Moderate aerobic exercise (MAE) provides a beneficial impact on intestinal immunity, but the action of MAE on TI-IgA generation under senescence conditions is unknown. This study aimed to determine the effects of long-term MAE on TI-IgA production in young (3 month old) BALB/c mice exercised until adulthood (6 months) or aging (24 months). Lamina propria (LP) from the small intestine was obtained to determine B cell and plasma cell sub-populations by flow cytometry and molecular factors related to class switch recombination [Thymic Stromal Lymphopoietin (TSLP), A Proliferation-Inducing Ligand (APRIL), B Cell Activating Factor (BAFF), inducible nitric oxide synthase (iNOS), and retinal dehydrogenase (RDH)] and the synthesis of IgA [α-chain, interleukin (IL)-6, IL-21, and Growth Factor-β (TGF-β)]; and epithelial cells evaluated IgA transitosis [polymeric immunoglobulin receptor (pIgR), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), IL-4] by the RT-qPCR technique. The results were compared with data obtained from sedentary age-matched mice. Statistical analysis was computed with ANOVA, and p < 0.05 was considered to be a statistically significant difference. Under senescence conditions, MAE promoted the B cell and IgA+ B cells and APRIL, which may improve the intestinal response and ameliorate the inflammatory environment associated presumably with the downmodulation of pro-inflammatory mediators involved in the upmodulation of pIgR expression. Data suggested that MAE improved IgA and downmodulate the cytokine pro-inflammatory expression favoring homeostatic conditions in aging.
摘要：
由微生物群激活的T细胞非依赖性（TI）途径导致低亲和力稳态IgA的产生，在肠道稳态中起关键作用。适度的有氧运动（MAE）对肠道免疫产生有益的影响，但是在衰老条件下MAE对TI-IgA生成的作用是未知的。这项研究旨在确定长期MAE对年轻（3月龄）BALB/c小鼠运动至成年（6个月）或衰老（24个月）的TI-IgA产生的影响。通过流式细胞术和与类别转换重组相关的分子因素，从小肠获得固有层（LP）以确定B细胞和浆细胞亚群[胸腺基质淋巴细胞生成素（TSLP），增殖诱导配体(APRIL),B细胞激活因子(BAFF),诱导型一氧化氮合酶（iNOS），和视网膜脱氢酶（RDH）]和IgA[α链的合成，白细胞介素（IL）-6，IL-21，和生长因子-β（TGF-β）];和上皮细胞评估IgA变性[聚合免疫球蛋白受体（pIgR），肿瘤坏死因子-α(TNF-α),干扰素-γ（IFN-γ），IL-4]通过RT-qPCR技术。将结果与从久坐的年龄匹配的小鼠获得的数据进行比较。统计分析用方差分析计算，并且p<0.05被认为是统计学上显著的差异。在衰老条件下，MAE促进B细胞和IgA+B细胞和APRIL，这可能会改善肠道反应并改善可能与pIgR表达上调相关的促炎介质下调相关的炎症环境。数据表明，MAE改善了IgA并下调了细胞因子促炎表达，有利于衰老中的稳态状况。
