PDF(Pubmed)
Abstract：
Osteoporosis in menopausal women requires alternatives to current medications, considering their adverse effects. In this context, probiotics and isoflavone products are promising dietary interventions. The objective of our study was to examine the impacts of Lactobacillus acidophilus and its combination with daidzein and tempeh on calcium status, calcium transporters, and bone metabolism biomarkers in a post-menopausal osteoporotic rat model. A total of 48 female Wistar rats were exposed to a two-stage experiment involving calcium deficit induction and subsequent dietary interventions across six groups. Calcium levels, the gene expression of TRPV5 and TRPV6 calcium transporters, bone histopathology, serum bone metabolism markers, and blood biochemistry were evaluated. The results revealed that, while decreasing serum calcium levels, the groups that received the probiotic L. acidophilus and isoflavone combination exhibited increased bone metabolism biomarkers and decreased calcium transporter expressions, akin to the effects of bisphosphonate. Additionally, significant improvements in bone histopathology were observed in these groups. However, the group receiving probiotic L. acidophilus alone did not exhibit significant changes in bone resorption biomarkers, calcium transporter expression, or various blood parameters. Meanwhile, the combination of probiotic L. acidophilus with tempeh positively influenced hematological parameters and reduced cholesterol and triglyceride levels, but it led to elevated blood glucose levels. Correlation analyses highlighted associations between serum calcium levels, calcium transporter expression, and bone metabolism biomarkers. In conclusion, our findings suggest that the daily consumption of probiotic L. acidophilus in combination with isoflavone products may improve bone health in ovariectomized rats, warranting further research to elucidate potential interactions with other nutrients.
摘要：
更年期妇女的骨质疏松症需要替代目前的药物，考虑到它们的不利影响。在这种情况下，益生菌和异黄酮产品是有前途的饮食干预措施。我们研究的目的是检查嗜酸乳杆菌及其与大豆苷元和豆蔻的组合对钙状态的影响，钙转运蛋白，绝经后骨质疏松大鼠模型的骨代谢生物标志物。共有48只雌性Wistar大鼠接受了两阶段实验，涉及六组的钙缺乏诱导和随后的饮食干预。钙含量,TRPV5和TRPV6钙转运体的基因表达，骨组织病理学,血清骨代谢标志物，并对血液生化进行了评价。结果显示,同时降低血清钙水平，接受益生菌嗜酸乳杆菌和异黄酮组合的组表现出增加的骨代谢生物标志物和减少的钙转运体表达，类似于双膦酸盐的作用。此外,在这些组中观察到骨组织病理学的显著改善.然而,仅接受益生菌嗜酸乳杆菌的组没有表现出骨吸收生物标志物的显著变化，钙转运体表达，或各种血液参数。同时,益生菌嗜酸乳杆菌与豆蔻的组合积极影响血液学参数和降低胆固醇和甘油三酯水平，但它导致血糖水平升高。相关分析强调了血清钙水平之间的关联，钙转运体表达，和骨代谢生物标志物。总之,我们的研究结果表明，每天食用益生菌嗜酸乳杆菌与异黄酮产品的组合可以改善卵巢切除大鼠的骨骼健康，需要进一步研究以阐明与其他营养素的潜在相互作用。
