PDF(Pubmed)
Abstract：
The Pichia kluyveri, a proliferation commonly found in Sichuan pickles (SCPs), can accelerate the growth and reproduction of spoilage bacteria, causing off-odor development and decay. Although D-limonene, a common natural preservative, effectively restricts P. kluyveri, its inhibitory mechanism remains unclear. This study aimed to elucidate this molecular mechanism by investigating the impact on basic P. kluyveri metabolism. The findings revealed that D-limonene inhibited P. kluyveri growth and disrupted the transcription of the genes responsible for encoding the enzymes involved in cell wall and membrane synthesis, oxidative phosphorylation, glycolysis, and the tricarboxylic acid (TCA) cycle pathway. The results indicated that these events disrupted crucial metabolism such as cell wall and membrane integrity, adenosine triphosphate (ATP) synthesis, and reactive oxygen species (ROS) balance. These insights provided a comprehensive understanding of the inhibitory effect of D-limonene on the growth and reproduction of P. kluyveri while highlighting its potential application in the SCP industry.
摘要：
PichiaKluyveri,一种常见于四川泡菜(SCP)的增殖，可以加速腐败菌的生长和繁殖，导致异味的发展和腐烂。虽然D-柠檬烯,一种常见的天然防腐剂，有效地限制了P.kluyveri,其抑制机制尚不清楚。本研究旨在通过研究对克鲁维氏疟原虫基本代谢的影响来阐明这一分子机制。研究结果表明，D-柠檬烯抑制了P.kluyveri的生长，并破坏了负责编码与细胞壁和膜合成有关的酶的基因的转录，氧化磷酸化，糖酵解,和三羧酸（TCA）循环途径。结果表明，这些事件破坏了关键的代谢，如细胞壁和膜的完整性，三磷酸腺苷(ATP)合成,和活性氧（ROS）平衡。这些见解提供了对D-柠檬烯对P.kluyveri生长和繁殖的抑制作用的全面了解，同时突出了其在SCP行业的潜在应用。
