PDF(Pubmed)
Abstract：
Escherichia coli (E. coli) was among the first organisms to have its complete genome published (Genome Sequence of E. coli 1997 Science). It is used as a model system in microbiology research. E. coli can cause life-threatening illnesses, particularly in children and the elderly. Possible contamination by the bacteria also results in product recalls, which, alongside the potential danger posed to individuals, can have significant financial consequences. We report the detection of live Escherichia coli (E. coli) in liquid samples using a biosensor based on a field-effect transistor (FET) biosensor with B/N co-coped reduced graphene oxide (rGO) gel (BN-rGO) as the transducer material. The FET was functionalized with antibodies to detect E. coli K12 O-antigens in phosphate-buffered saline (PBS). The biosensor detected the presence of planktonic E. coli bacterial cells within a mere 2 min. The biosensor exhibited a limit of detection (LOD) of 10 cells per sample, which can be extrapolated to a limit of detection at the level of a single cell per sample and a detection range of at least 10-108 CFU/mL. The selectivity of the biosensor for E. coli was demonstrated using Bacillus thuringiensis (B. thuringiensis) as a sample contaminant. We also present a comparison of our functionalized BN-rGO FET biosensor with established detection methods of E. coli k12 bacteria, as well as with state-of-the-art detection mechanisms.
摘要：
大肠杆菌(E.大肠杆菌）是最早发表其完整基因组的生物之一（大肠杆菌基因组序列1997科学）。它被用作微生物学研究中的模型系统。大肠杆菌会导致危及生命的疾病，尤其是儿童和老人。可能被细菌污染也会导致产品召回，which,除了对个人构成的潜在危险之外，可能会产生重大的财务后果。我们报告了活大肠杆菌的检测(E.大肠杆菌）在液体样品中使用基于场效应晶体管（FET）生物传感器的生物传感器，以B/N共处理的还原氧化石墨烯（rGO）凝胶（BN-rGO）作为换能器材料。用抗体官能化FET以检测磷酸盐缓冲盐水(PBS)中的大肠杆菌K12〇-抗原。生物传感器在仅仅2分钟内检测到浮游大肠杆菌细菌细胞的存在。生物传感器的检测限（LOD）为每个样品10个细胞，可以外推到每个样品单个细胞水平的检测极限和至少10-108CFU/mL的检测范围。使用苏云金芽孢杆菌（B.苏云金）作为样品污染物。我们还介绍了我们的功能化BN-rGOFET生物传感器与已建立的大肠杆菌k12细菌检测方法的比较，以及最先进的检测机制。
