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Abstract：
The reproductive performance of horse sperm and donkey sperm has been reported to differ. Sperm proteins play a crucial role in sperm viability and fertility. Although differences between species are known, no prior study has investigated disparities in the sperm proteome between horses and donkeys. Therefore, this study characterized and compared the sperm proteomes of horses and donkeys using 4D-DIA mass spectrometry technology. We identified 3436 proteins in horse sperm and 3404 proteins in donkey sperm. Of these, 3363 proteins were expressed in both horse and donkey sperm, with 73 proteins being specifically expressed in horse sperm, and 41 in donkey sperm. According to data analysis, donkeys exhibited a greater percentage of motility and progressive movement in straight-line sperm than horses, as well as lower percentages of static and slow sperm than horses. Joint analysis of the results from the horse and donkey sperm proteomes and their CEROS II-read parameters demonstrated a possible association between sperm proteins and their sperm viability patterns. These findings suggest that there are discrepancies in the expression levels and protein compositions of horse and donkey sperm and that certain specific proteins may be responsible for the differences in performance between these two species.
摘要：
据报道，马精子和驴精子的繁殖性能不同。精子蛋白在精子活力和生育力中起着至关重要的作用。虽然物种之间的差异是已知的，先前没有研究调查马和驴之间精子蛋白质组的差异。因此,这项研究使用4D-DIA质谱技术表征和比较了马和驴的精子蛋白质组。我们鉴定了马精子中的3436种蛋白质和驴精子中的3404种蛋白质。其中,3363蛋白在马和驴精子中表达，73种蛋白质在马精子中特异性表达，和41只驴精子。根据数据分析,驴在直线精子中表现出比马更高的运动和渐进运动百分比，以及静态和慢速精子的百分比低于马。对马和驴精子蛋白质组及其CEROSII读取参数的结果的联合分析表明，精子蛋白与其精子活力模式之间可能存在关联。这些发现表明，马和驴精子的表达水平和蛋白质组成存在差异，并且某些特定的蛋白质可能是这两个物种之间性能差异的原因。
