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Abstract：
Lycium barbarum L., an important functional food in China, has antioxidant and antiaging activity. However, the exact antioxidant activity mechanism of Lycium barbarum extracts (LBE) is not well understood. Therefore, a carbendazim (CBZ)-induced PC12 cell injury model was constructed and vitrificated to study the antioxidant activity of fresh LBE on the basis of extraction parameter optimization via the full factorial design of experiments (DOE) method. The results showed that the pretreatment of PC12 cells with LBE could reduce the reactive oxygen species (ROS) level by 14.6% and inhibited the mitochondrial membrane potential (MMP) decline by 12.0%. Furthermore, the integrated analysis revealed that LBE played an antioxidant role by activating oxidative phosphorylation (OXPHOS) and restoring MMP, maintaining the tricarboxylic acid (TCA) cycle stability, and regulating the GSH metabolic pathway. The results of the present study provide new ideas for the understanding of the antioxidant function of LBE from a global perspective.
摘要：
枸杞,是中国重要的功能性食品，具有抗氧化和抗衰老活性。然而,枸杞提取物（LBE）的确切抗氧化活性机制尚不清楚。因此,采用全析因设计(DOE)法,在提取参数优化的基础上,建立多菌灵(CBZ)诱导的PC12细胞损伤模型,并对其抗氧化活性进行研究。结果表明,LBE预处理PC12细胞可使活性氧(ROS)水平降低14.6%,抑制线粒体膜电位(MMP)下降12.0%。此外，综合分析表明，LBE通过激活氧化磷酸化（OXPHOS）和恢复MMP发挥抗氧化作用，保持三羧酸（TCA）循环稳定性，调节GSH代谢途径。本研究结果为从全球角度理解LBE的抗氧化功能提供了新的思路。
