PDF(Pubmed)
Abstract：
Cyclophosphamide (CTX) is the most commonly used effective alkylating drug in cancer treatment, but its use is restricted because its toxic side effect causes testicular toxicity. CTX disrupts the tissue redox and antioxidant balance and the resulting tissue damage causes oxidative stress. In our study based on this problem, kefir against CTX-induced oxidative stress and testicular toxicity were investigated. Rats were divided into 6 groups: control, 150 mg/kg CTX, 5 and 10 mg/kg kefir, 5 and 10 mg/kg kefir + 150 CTX. While the fermented kefirs were mixed and given to the rats for 12 days, CTX was given as a single dose on the 12th day of the experiment. Testis was scored according to spermatid density, giant cell formation, cells shed into tubules, maturation disorder, and atrophy. According to our biochemical findings, the high levels of total oxidant status (TOS), and the low levels of total antioxidant status (TAS) in the CTX group, which are oxidative stress markers, indicate the toxic effect of CTX, while the decrease in TOS levels and the increase in TAS levels in the kefir groups indicate the protective effect of kefir. In the CTX-administered group, tubules with impaired maturation and no spermatids were observed in the transverse section of the testicle, while in the kefir groups, the presence of near-normal tubule structures and tubule lumens despite CTX showed the protective effect of kefir. In our study, it was observed that kefir had a protective and curative effect on CTX-induced toxicity and oxidative stress and could be a strong protector.
摘要：
环磷酰胺（CTX）是癌症治疗中最常用的有效烷化药物，但它的使用受到限制，因为它的毒副作用导致睾丸毒性。CTX破坏组织氧化还原和抗氧化剂平衡,并且所产生的组织损伤引起氧化应激。在我们基于这个问题的研究中，研究了开菲尔对CTX诱导的氧化应激和睾丸毒性的影响。大鼠分为6组:对照组,150毫克/千克CTX，5和10毫克/千克开菲尔，5和10mg/kg的kefir+150CTX。在将发酵的凯夫混合并给予大鼠12天的同时，CTX在实验的第12天作为单剂量给予。根据精子细胞密度对睾丸进行评分，巨细胞形成,细胞脱落到小管中，成熟障碍,和萎缩。根据我们的生化发现,高水平的总氧化剂状态(TOS)，和低水平的总抗氧化剂状态（TAS）在CTX组，它们是氧化应激标志物，表明CTX的毒性作用，而开菲尔组中TOS水平的降低和TAS水平的增加表明了开菲尔的保护作用。在CTX给药组,在睾丸的横截面中观察到成熟受损且没有精子细胞的小管，在开菲尔集团，尽管有CTX，但仍存在接近正常的小管结构和小管腔，显示了开菲尔的保护作用。在我们的研究中,观察到开菲尔对CTX引起的毒性和氧化应激具有保护和疗效，并且可能是一种强大的保护剂。
