Abstract：
Bacterial infections already pose a significant threat to skin wounds, especially in diabetic patients who have difficulty healing wounds. However, wound or bacterial infections are known to produce excess reactive oxygen species (ROS), and hypoxia may further hinder wound healing and the development of chronic wounds. In this study, a multifunctional hydrogel for ROS scavenging and bacterial inhibition was developed by cross-linking polyvinyl alcohol (PVA) and sodium alginate (SA) with graphene oxide (GO) loaded with silver-platinum hybrid nanoparticles (GO@Ag-Pt). The PVA/SA hydrogel loaded with GO@Ag-Pt exhibited the ability to scavenge different types of ROS, generate O2, and kill a broad spectrum of bacteria in vitro. The silver-platinum hybrid nanoparticles significantly increased the antibacterial ability against Escherichia coli and Staphylococcus aureus compared with silver nanoparticles (AgNps). GO@Ag-Pt loaded hydrogel was effective in treating infections caused by S.aureus, thereby significantly promoting wound healing during the inflammatory phase. Hydrogel therapy significantly reduced the level of ROS and alleviated inflammation levels. Notably, our ROS-scavenging, antibacterial hydrogels can be used to effectively treat various types of wounds, including difficult-to-heal diabetic wounds with bacterial infections. Thus, this study proposes an effective strategy for various chronic wound healing based on ROS clearance and bacteriostatic hydrogels.
摘要：
细菌感染已经对皮肤伤口构成重大威胁，尤其是在伤口难以愈合的糖尿病患者中。然而,已知伤口或细菌感染会产生过量的活性氧（ROS），缺氧可能进一步阻碍伤口愈合和慢性伤口的发展。在这项研究中,通过将聚乙烯醇（PVA）和海藻酸钠（SA）与负载有银-铂杂化纳米颗粒（GO@Ag-Pt）的氧化石墨烯（GO）交联，开发了一种用于ROS清除和细菌抑制的多功能水凝胶。负载GO@Ag-Pt的PVA/SA水凝胶表现出清除不同类型ROS的能力，产生O2，并在体外杀死广谱细菌。与银纳米粒子（AgNps）相比，银-铂杂化纳米粒子显着提高了对大肠杆菌和金黄色葡萄球菌的抗菌能力。GO@Ag-Pt负载水凝胶可有效治疗金黄色葡萄球菌引起的感染，从而在炎症阶段显著促进伤口愈合。水凝胶疗法显著降低了ROS水平并减轻了炎症水平。值得注意的是,我们的ROS清除，抗菌水凝胶可用于有效治疗各种类型的伤口，包括难以治愈的糖尿病伤口和细菌感染。因此，这项研究提出了一种基于ROS清除和抑菌水凝胶的各种慢性伤口愈合的有效策略。
