PDF(Pubmed)
Abstract：
A LOx-based electrochemical biosensor for high-level lactate determination was developed. For the construction of the biosensor, chitosan and Nafion layers were integrated by using a spin coating procedure, leading to less porous surfaces in comparison with those recorded after a drop casting procedure. The analytical performance of the resulting biosensor for lactate determination was evaluated in batch and flow regime, displaying satisfactory results in both modes ranging from 0.5 to 20 mM concentration range for assessing the lactic acidosis. Finally, the lactate levels in raw serum samples were estimated using the biosensor developed and verified with a blood gas analyzer. Based on these results, the biosensor developed is promising for its use in healthcare environment, after its proper miniaturization. A pH probe based on common polyaniline-based electrochemical sensor was also developed to assist the biosensor for the lactic acidosis monitoring, leading to excellent results in stock solutions ranging from 6.0 to 8.0 mM and raw plasma samples. The results were confirmed by using two different approaches, blood gas analyzer and pH-meter. Consequently, the lactic acidosis monitoring could be achieved in continuous flow regime using both (bio)sensors.
摘要：
开发了基于LOx的用于高水平乳酸测定的电化学生物传感器。为了构建生物传感器，壳聚糖和Nafion层通过使用旋涂程序整合，与滴铸程序后记录的表面相比，导致多孔表面更少。在分批和流动状态下评估了所得用于乳酸测定的生物传感器的分析性能，在评估乳酸性酸中毒的两种模式下，在0.5至20mM浓度范围内都显示出令人满意的结果。最后,使用开发的生物传感器估算原始血清样本中的乳酸水平，并通过血气分析仪进行验证。基于这些结果,开发的生物传感器有望在医疗保健环境中使用，在其适当的小型化之后。还开发了基于普通聚苯胺电化学传感器的pH探针，以辅助生物传感器监测乳酸酸中毒，导致在6.0至8.0mM的原液和原始血浆样品中获得优异的结果。通过使用两种不同的方法证实了结果，血气分析仪和pH计。因此,乳酸性酸中毒监测可以使用两种（生物）传感器在连续流动状态下实现。
