Abstract：
This report describes the characterization of Burkholderia cenocepacia isolates belonging to sequence type (ST)-250, detected in eight patients with cystic fibrosis (CF) in Switzerland. We retrospectively analyzed 18 isolates of B. cenocepacia ST-250 isolated between 2003 and 2015 by whole-genome sequencing and evaluated clinical and epidemiological data. Single nucleotide polymorphism analysis of the B.°cenocepacia ST-250 lineage showed that the isolates from all patients cluster tightly, suggesting that this cluster has a recent common ancestor. Epidemiological investigations showed that six out of eight patients acquired B.°cenocepacia ST-250 in the years 2001-2006, where participation in CF summer camps was common. Two patients were siblings. Genomic relatedness of the B. cenocepacia ST-250 isolates supported transmission by close contact, however, a common source or nosocomial routes cannot be excluded. With respect to the fatal outcome in six patients, our study shows the importance of infection control measurements in CF patients with B.°cenocepacia.
摘要：
该报告描述了在瑞士的八名囊性纤维化（CF）患者中检测到的属于序列型（ST）-250的伯克霍尔德氏菌分离株的特征。我们通过全基因组测序对2003年至2015年间分离的18株隐血芽孢杆菌ST-250进行了回顾性分析，并评估了临床和流行病学数据。B.°cincepaciaST-250谱系的单核苷酸多态性分析表明，所有患者的分离株紧密聚集，表明这个集群有一个最近的共同祖先。流行病学调查显示，在2001-2006年期间，八名患者中有六名获得了B.°cenocepaciaST-250，在那里参加CF夏令营很普遍。两名患者是兄弟姐妹。通过密切接触支持传播的隐血芽孢杆菌ST-250分离株的基因组相关性，然而,不能排除常见的来源或医院途径。关于六名患者的致命结局，我们的研究表明，在患有B.°ciocepacia的CF患者中进行感染控制测量的重要性。
