Abstract：
Molecular farming, also known as plant molecular farming (PMF), is a technique that involves using plants and plant cells as bioreactors to produce recombinant proteins. This is a cost-effective and sustainable way of producing large quantities of proteins for various applications, including pharmaceuticals, vaccines, and industrial enzymes. An endogenous or exogenous signal peptide (SP) is flanked at the N-terminal for recombinant protein targeting and storage. These SPs are responsible for guiding the recombinant protein products to the correct destination within the plant cell or facilitating their secretion into the extracellular space. In this chapter, we will give a brief introduction of the current PMF research outcomes supported by the basic study of vesicle trafficking and protein secretion, mainly introducing the bright yellow 2 (BY-2) cell-based secretion pathway and its associated protocols according to our study of recombinant human iduronidase.
摘要：
分子农业,也被称为植物分子农业(PMF)，是一种使用植物和植物细胞作为生物反应器生产重组蛋白的技术。这是一种具有成本效益和可持续的方法，可以生产大量用于各种应用的蛋白质，包括药品,疫苗,和工业酶。内源性或外源性信号肽(SP)侧翼在N-末端用于重组蛋白靶向和储存。这些SP负责将重组蛋白产物引导到植物细胞内的正确目的地或促进其分泌到细胞外空间中。在这一章中,我们将简要介绍由囊泡运输和蛋白质分泌的基础研究支持的当前PMF研究成果，根据我们对重组人艾杜糖醛酸酶的研究，主要介绍了亮黄2（BY-2）细胞分泌途径及其相关方案。
