PDF(Pubmed)
Abstract：
The gut microbiota significantly contributes to human health and well-being. The aim of this study was to evaluate the stability and resilience of a consortium composed of three next-generation probiotics (NGPs) candidates originally found in the human gut. The growth patterns of Akkermansia muciniphila, Bacteroides thetaiotaomicron, and Faecalibacterium prausnitzii were studied both individually and consortium. The growth kinetics of Akkermansia muciniphila (A. muciniphila), Bacteroides thetaiotaomicron (B. thetaiotaomicron), and Faecalibacterium prausnitzii (F. prausnitzii) were characterized both individually and in consortium using isothermal microcalorimetry and 16S ribosomal RNA next-generation sequencing. The consortium reached stability after three passages and demonstrated resilience to changes in its initial composition. The concentration of butyrate produced was nearly twice as high in the consortium compared to the monoculture of F. prausnitzii. The experimental conditions and methodologies used in this article are a solid foundation for developing further complex consortia.
摘要：
肠道微生物群显著有助于人类健康和福祉。这项研究的目的是评估由最初在人类肠道中发现的三种下一代益生菌（NGP）候选物组成的财团的稳定性和弹性。Akkermansiamuciniphila的生长模式，拟杆菌，和prausnitzii粪杆菌分别进行了研究和研究。Akkermansiamuciniphia的生长动力学（A.粘液虫)，拟杆菌（B.thetaiotaomicron)，和普氏粪杆菌(F.prausnitzii）使用等温微量热法和16S核糖体RNA下一代测序分别和财团进行了表征。该财团经过三段传代后达到了稳定性，并表现出了对其初始组成变化的抵抗力。与普劳斯尼齐的单一培养物相比，该财团中产生的丁酸盐浓度几乎是后者的两倍。本文使用的实验条件和方法为进一步发展复杂联盟奠定了坚实的基础。
