Abstract：
The current study aimed to explore the genotoxic impacts of the insecticide acetamiprid (ACP) on the myocardium and assess the ameliorative role of resveratrol (RSV). Male rats (10/group) were treated via oral route for 90 days: control; ACP (25 mg/kg); RSV (20 mg/kg); ACP+RSV. Peripheral blood micronucleus test, oxidative stress analysis, comet assay, 8-hydroxydeoxyguanosine and gene expression assessment were performed. The findings revealed that ACP has myocardial genotoxic effects, as demonstrated by increased micronucleus and 8-hydroxydeoxyguanosine formation and increased all comet parameters. Oxidative stress analysis demonstrated that ACP elevated H2O2 and NO levels while decreasing catalase and GST activities. Acetamiprid dysregulated the expression of genes related to oxidative stress and DNA damage response. However, RSV co-treatment resulted in significant protection against these genotoxic impacts. Resveratrol reduced DNA damage and restored the oxidative balance in the myocardium. Moreover, RSV modulated the Nrf2/HO-1 and Atm/P53 pathways, potentiating antioxidant defense and DNA repair.
摘要：
本研究旨在探讨杀虫剂啶虫脒（ACP）对心肌的遗传毒性影响，并评估白藜芦醇（RSV）的改善作用。雄性大鼠(10只/组)通过口服途径治疗90天:对照;ACP(25mg/kg);RSV(20mg/kg);ACP+RSV。外周血微核试验,氧化应激分析，彗星试验，进行8-羟基脱氧鸟苷和基因表达评估。研究结果表明，ACP具有心肌遗传毒性作用，如增加的微核和8-羟基脱氧鸟苷形成所证明的，并增加了所有彗星参数。氧化应激分析表明，ACP升高了H2O2和NO水平，同时降低了过氧化氢酶和GST活性。啶虫脒失调与氧化应激和DNA损伤反应相关基因的表达。然而,RSV共同治疗导致对这些基因毒性影响的显著保护。白藜芦醇减少DNA损伤并恢复心肌中的氧化平衡。此外，RSV调节Nrf2/HO-1和Atm/P53通路，增强抗氧化防御和DNA修复。
