Abstract：
To understand the function of cells such as neurons within an organism, it can be instrumental to inhibit cellular function, or to remove the cell (type) from the organism, and thus to observe the consequences on organismic and/or circuit function and animal behavior. A range of approaches and tools were developed and used over the past few decades that act either constitutively or acutely and reversibly, in systemic or local fashion. These approaches make use of either drugs or genetically encoded tools. Also, there are acutely acting inhibitory tools that require an exogenous trigger like light. Here, we give an overview of such methods developed and used in the nematode Caenorhabditis elegans.
摘要：
为了理解生物体内的神经元等细胞的功能，它可以抑制细胞功能，或从生物体中去除细胞（类型），从而观察对生物和/或电路功能和动物行为的后果。在过去的几十年中，开发和使用了一系列方法和工具，这些方法和工具既可以构成也可以急剧和可逆地发挥作用，以系统或局部的方式。这些方法利用药物或基因编码的工具。此外，有急性作用的抑制工具，需要一个外生触发如光。这里,我们概述了在线虫秀丽隐杆线虫中开发和使用的此类方法。
