Abstract：
OBJECTIVE: To characterize the extracellular vesicle protein cargo in the aqueous humor and plasma of patients with ocular toxoplasmosis.
METHODS: Aqueous humor and plasma were collected from six patients with active ocular toxoplasmosis and six patients with cataract. Extracellular vesicles were isolated, and western blotting and mass spectrometry were performed for protein analysis.
RESULTS: All plasma samples from patients with ocular toxoplasmosis and cataract were positive for the tetraspanins CD63 and TSG101. However, the aqueous humor from patients with ocular toxoplasmosis was positive only for CD63. Sixty-seven new unreported proteins were identified in the aqueous humor and plasma of patients with the ocular toxoplasmosis and cataract. Of the 67 proteins, 10 and 7 were found only in the cataract and ocular toxoplasmosis groups, respectively. In general, these proteins were involved in immune system activation and retina homeostasis and were related to infections and retina-associated diseases.
CONCLUSIONS: The distinct protein signatures between ocular toxoplasmosis and cataract may be helpful in the differential diagnosis of ocular toxoplasmosis. However, more studies are needed to better understand the role of these proteins in the pathogenesis of ocular toxoplasmosis.
METHODS: Aqueous humor and plasma were collected from six patients with active ocular toxoplasmosis and six patients with cataract. Extracellular vesicles were isolated, and western blotting and mass spectrometry were performed for protein analysis.
RESULTS: All plasma samples from patients with ocular toxoplasmosis and cataract were positive for the tetraspanins CD63 and TSG101. However, the aqueous humor from patients with ocular toxoplasmosis was positive only for CD63. Sixty-seven new unreported proteins were identified in the aqueous humor and plasma of patients with the ocular toxoplasmosis and cataract. Of the 67 proteins, 10 and 7 were found only in the cataract and ocular toxoplasmosis groups, respectively. In general, these proteins were involved in immune system activation and retina homeostasis and were related to infections and retina-associated diseases.
CONCLUSIONS: The distinct protein signatures between ocular toxoplasmosis and cataract may be helpful in the differential diagnosis of ocular toxoplasmosis. However, more studies are needed to better understand the role of these proteins in the pathogenesis of ocular toxoplasmosis.
摘要：
目的：研究眼弓形虫病患者房水和血浆中细胞外囊泡蛋白的特征。
方法：收集6例活动性眼弓形虫病患者和6例白内障患者的房水和血浆。分离细胞外囊泡，进行蛋白质印迹和质谱分析。
结果：眼弓形虫病和白内障患者的所有血浆样本均为四跨膜蛋白CD63和TSG101阳性。然而,眼弓形虫病患者的房水仅CD63阳性.在眼弓形虫病和白内障患者的房水和血浆中鉴定出67种新的未报告蛋白质。在67种蛋白质中，10和7只在白内障和眼弓形虫病组中发现，分别。总的来说,这些蛋白参与免疫系统激活和视网膜稳态,并与感染和视网膜相关疾病有关.
结论：眼弓形虫病和白内障之间不同的蛋白质特征可能有助于眼弓形虫病的鉴别诊断。然而,需要更多的研究来更好地了解这些蛋白质在眼弓形虫病发病机制中的作用。
方法：收集6例活动性眼弓形虫病患者和6例白内障患者的房水和血浆。分离细胞外囊泡，进行蛋白质印迹和质谱分析。
结果：眼弓形虫病和白内障患者的所有血浆样本均为四跨膜蛋白CD63和TSG101阳性。然而,眼弓形虫病患者的房水仅CD63阳性.在眼弓形虫病和白内障患者的房水和血浆中鉴定出67种新的未报告蛋白质。在67种蛋白质中，10和7只在白内障和眼弓形虫病组中发现，分别。总的来说,这些蛋白参与免疫系统激活和视网膜稳态,并与感染和视网膜相关疾病有关.
结论：眼弓形虫病和白内障之间不同的蛋白质特征可能有助于眼弓形虫病的鉴别诊断。然而,需要更多的研究来更好地了解这些蛋白质在眼弓形虫病发病机制中的作用。
