Abstract：
The Astragalus grahamianus (AG) Royle ex. Benth is traditionally used for the treatment of various human disorders. The current research work is aimed to explore the neuroprotective anti-Parkinson effects of various fractions of Astragalus grahamianus (A. grahamianus). Fine powder of Astragalus grahamianus was extracted with 70% methanol and then fractionated with various solvents on the basis of polarity. Standard protocols were used to investigate the bioactive constituents present in the various plant fractions. In-vitro antioxidant potential of various fractions was checked using diverse free radicals. In-vivo rats model was used to determined the neuroprotective effects of methanol fraction of A. grahamianus. The results revealed that various fractions of A. grahamianus contain flavonoids, cardiac glycosides, steroids, gums, terpenes, proteins, and carbohydrates except chloroform fraction lake the presence of steroids, cardiac glycosides, gums and saponins, aqueous fraction of steroids, terpenoids, gums and saponins, n-Hexane fraction steroids, carbohydrates, alkaloids, gums and flavonoids. The highest amount of total phenolic contents was found in AGME (32.67 ± 2.3 mg GAE / g). The AGME also showed enhanced free radicals cations potential against DPPH, ABTS and H2O2, respectively. The correlation between AOA (antioxidant activity) and TPC (total phenolic contents) revealed to be substantial. Relative R2 values for ABTS, H2O2, and DPPH activity are 0.9974, 0.9845, and 0.9678, respectively. The in-vivo neuroprotective activities showed significant results. Our findings highlight significant antioxidant, and neuroprotective possessions of AGME attributed to powerful bioactive compounds.
摘要：
黄芪（AG）Royleex。Benth传统上用于治疗各种人类疾病。目前的研究工作旨在探索黄芪各个部位的神经保护性抗帕金森作用（A.grahamianus）。用70％甲醇提取黄芪细粉，然后根据极性用各种溶剂分馏。使用标准方案来研究存在于各种植物级分中的生物活性成分。使用不同的自由基检查了各种级分的体外抗氧化潜力。使用体内大鼠模型来确定A.grahamianus的甲醇部分的神经保护作用。结果表明，格拉哈米阿斯的各种级分都含有类黄酮，强心苷，类固醇,牙龈,萜烯,蛋白质，和碳水化合物，除了氯仿部分湖类固醇的存在，强心苷，口香糖和皂苷，类固醇的水性部分，萜类化合物,口香糖和皂苷，正己烷馏分类固醇,碳水化合物,生物碱，树胶和类黄酮。在AGME中发现总酚含量最高（32.67±2.3mgGAE/g）。AGME还显示出增强的自由基阳离子对DPPH的潜力，分别为ABTS和H2O2。显示AOA（抗氧化活性）和TPC（总酚含量）之间的相关性很大。ABTS的相对R2值，H2O2和DPPH活性分别为0.9974、0.9845和0.9678。体内神经保护活性显示出显著的结果。我们的发现强调了重要的抗氧化剂，和AGME的神经保护财产归因于强大的生物活性化合物。
