PDF(Pubmed)
Abstract：
Tendinopathy is one of the most frequent musculoskeletal disorders characterized by sustained tissue inflammation and oxidative stress, accompanied by extracellular matrix remodeling. Patients suffering from this pathology frequently experience pain, swelling, stiffness, and muscle weakness. Current pharmacological interventions are based on nonsteroidal anti-inflammatory drugs; however, the effectiveness of these strategies remains ambiguous. Accumulating evidence supports that oral supplementation of natural compounds can provide preventive, and possibly curative, effects. Vitamin C (Vit C), collagen peptides (Coll), resveratrol (Res), and astaxanthin (Asx) were reported to be endowed with potential beneficial effects based on their anti-inflammatory and antioxidant activities. Here, we analyzed the efficacy of a novel combination of these compounds (Mix) in counteracting proinflammatory (IL-1β) and prooxidant (H2O2) stimuli in human tenocytes. We demonstrated that Mix significantly impairs IL-6-induced IL-1β secretion, NF-κB nuclear translocation, and MMP-2 production; notably, a synergistic effect of Mix over the single compounds could be observed. Moreover, Mix was able to significantly counteract H2O2-triggered ROS production. Together, these results point out that Mix, a novel combination of Vit C, Coll, Resv, and Asx, significantly impairs proinflammatory and prooxidant stimuli in tenocytes, mechanisms that contribute to the onset of tendinopathies.
摘要：
肌腱病是最常见的肌肉骨骼疾病之一，其特征是持续的组织炎症和氧化应激。伴有细胞外基质重塑。患有这种病理的患者经常经历疼痛，肿胀,刚度,肌肉无力。目前的药物干预措施是基于非甾体抗炎药;然而，这些策略的有效性仍然模棱两可。越来越多的证据表明，口服补充天然化合物可以提供预防，可能是治愈的，效果。维生素C（维生素C），胶原蛋白肽（Coll），白藜芦醇（Res），据报道，虾青素（Asx）具有抗炎和抗氧化活性，具有潜在的有益作用。这里,我们分析了这些化合物（Mix）的新型组合在对抗人腱细胞促炎（IL-1β）和促氧化剂（H2O2）刺激中的功效。我们证明Mix显著损害IL-6诱导的IL-1β分泌，NF-κB核易位,和MMP-2的生产；特别是，可以观察到混合物对单一化合物的协同作用。此外，混合物能够显著抵消H2O2触发的ROS产生。一起,这些结果表明，混合，VitC的新颖组合，Coll,Resv,Asx,显着损害肌腱细胞的促炎和促氧化剂刺激，导致肌腱病发作的机制。
