Abstract：
Background: This study aimed to investigate the comprehensive expression profile of cancer stem cell (CSC)-related genes and construct a prognostic signature for overall survival (OS) prediction in high-risk Wilms\' tumor (WT). Materials and methods: Gene expression and survival data from 120 high-risk WT cases in the Therapeutically Applicable Research to Generate Effective Treatments (TARGET)-WT were used. Results: In total, 229 CSC-related genes were found to be significantly dysregulated in WT compared to tumor-adjacent normal tissues, among which 34 were associated with OS. Using LASSO regression, a 22-gene signature was developed, which exhibited excellent performance in 3-, 5-, and 10-year OS predictions (AUC > 0.86). The high-risk score group showed markedly poorer OS compared to the low-risk score group (median separation, HR = 6.41, 95% CI: 3.18-12.92, p = 3.2e - 9). The 22-gene signature was an independent prognostic factor for OS (HR = 5.086, 95% CI: 3.019-8.568, p < 0.001). Conclusion: This study identified a robust prognostic signature that can effectively support OS prediction.
摘要：
背景：本研究旨在研究癌症干细胞（CSC）相关基因的综合表达谱，并构建高风险肾母细胞瘤（WT）的总体生存（OS）预测的预后标志。材料和方法:使用来自产生有效治疗的治疗应用研究(TARGET)-WT中的120个高风险WT病例的基因表达和存活数据。结果：总的来说，与肿瘤附近的正常组织相比，在WT中发现229个CSC相关基因显着失调，其中34个与OS相关。使用LASSO回归，开发了22个基因签名，在3-，5-,和10年操作系统预测(AUC>0.86)。与低风险评分组相比，高风险评分组显示出明显较差的OS（中位数分离，HR=6.41，95%CI：3.18-12.92，p=3.2e-9）。22基因标记是OS的独立预后因素(HR=5.086,95%CI:3.019-8.568,p<0.001)。结论：这项研究确定了一个强大的预后特征，可以有效地支持OS预测。
