PDF(Pubmed)
Abstract：
Ephedra is one of the many medicinal herbs that have been used as folk/traditional medicine in Jordan and other countries to cure various illnesses. Plants of this genus are well known for their antioxidant and antibacterial properties. In this study, three different solvents were used to obtain Ephedra extracts. When evaluated, the Ephedra alata Decne ethanolic extract reportedly had the greatest levels of total phenolic compounds (TPC) and total flavonoid compounds (TFC). The aqueous extracts displayed the highest antioxidant activity in the DPPH and ABTS assays, demonstrating their considerable capacity to neutralize free radicals. However, when evaluated using the FRAP method, the acetone extracts showed the strongest antioxidant activity, indicating their high reducing power. LC-MS/MS, a potent method of analysis that combines the liquid chromatographic separation properties with mass spectrometry detection and identification capabilities, was used in this study to detect and measure phytochemical content of a total of 24 phenolic compounds and 16 terpene compounds present in the extracts of Ephedra alata Decne. Various concentrations of these chemicals were found in these extracts. The extracts\' inhibitory effects on albumin denaturation and alpha-amylase activity were also assessed; the findings demonstrated the potentials of these extracts as anti-inflammatory and anti-diabetic medicines, with the acetone extract having the lowest IC50 values in the concomitant tests (306.45 µg/ml and 851.23 µg/ml, respectively). Furthermore, the lowest IC50 value (of 364.59 ± 0.45 µg/ml) for the 80% ethanol extract demonstrated that it has the strongest antiproliferative impact regarding the MDA-MB-231 breast cancer cell line. This finding indicates that this particular extract can be potentially used to treat cancer.
摘要：
麻黄是在约旦和其他国家被用作民间/传统医学来治疗各种疾病的许多草药之一。该属植物以其抗氧化和抗菌特性而闻名。在这项研究中,使用三种不同的溶剂来获得麻黄提取物。评估时，据报道，麻黄decne乙醇提取物的总酚类化合物（TPC）和总类黄酮化合物（TFC）含量最高。水提物在DPPH和ABTS试验中表现出最高的抗氧化活性，展示了他们相当大的中和自由基的能力。然而,当使用FRAP方法评估时，丙酮提取物表现出最强的抗氧化活性，表明他们的高还原能力。LC-MS/MS,一种有效的分析方法，将液相色谱分离特性与质谱检测和识别功能相结合，在这项研究中，用于检测和测量麻黄提取物中总共存在的24种酚类化合物和16种萜类化合物的植物化学物质含量。在这些提取物中发现了各种浓度的这些化学物质。还评估了提取物对白蛋白变性和α-淀粉酶活性的抑制作用;研究结果表明了这些提取物作为抗炎和抗糖尿病药物的潜力。丙酮提取物在伴随试验中具有最低的IC50值（306.45µg/ml和851.23µg/ml，分别)。此外，80％乙醇提取物的最低IC50值（364.59±0.45µg/ml）表明，它对MDA-MB-231乳腺癌细胞系具有最强的抗增殖作用。这一发现表明这种特定的提取物可以潜在地用于治疗癌症。
