PDF(Pubmed)
Abstract：
Aposematic coloration offers an opportunity to explore the molecular mechanisms underlying canalization. In this study, the role of epigenetic regulation underlying robustness was explored in the aposematic coloration of the milkweed bug, Oncopeltus fasciatus. Polycomb (Pc) and Enhancer of zeste (E(z)), which encode components of the Polycomb repressive complex 1 (PRC1) and PRC2, respectively, and jing, which encodes a component of the PRC2.2 subcomplex, were knocked down in the fourth instar of O. fasciatus. Knockdown of these genes led to alterations in scutellar morphology and melanization. In particular, when Pc was knocked down, the adults developed a highly melanized abdomen, head and forewings at all temperatures examined. In contrast, the E(z) and jing knockdown led to increased plasticity of the dorsal forewing melanization across different temperatures. Moreover, jing knockdown adults exhibited increased plasticity in the dorsal melanization of the head and the thorax. These observations demonstrate that histone modifiers may play a key role during the process of canalization to confer robustness in the aposematic coloration.
摘要：
反向着色为探索管道化的分子机制提供了机会。在这项研究中,在乳草虫的配相着色中探索了表观遗传调控潜在稳健性的作用，筋膜盘。Polycomb（Pc）和zeste增强剂（E（z）），分别编码Polycomb抑制复合物1（PRC1）和PRC2的成分，而京,它编码PRC2.2亚复合物的一个组成部分，被击倒在O.fasciatus的第四龄。这些基因的敲除导致小脑形态和黑化的改变。特别是,当PC被撞倒时,成年人的腹部高度黑化，头部和前爪在所有温度检查。相比之下,在不同温度下，E（z）和jing击倒导致背前黑化的可塑性增加。此外，精敲成虫在头部和胸部的背侧黑化表现出增加的可塑性。这些观察结果表明，组蛋白修饰剂可能在管道化过程中起关键作用，以赋予配相着色的鲁棒性。
