Abstract：
Cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CMs) offer an attractive platform to evaluate the mechanisms of cardiovascular-related incidents and to develop and test new drugs for heart diseases. This work focuses on the comparison of two hiPSC-CM differentiation protocols: the GiWi method based on temporal modulation of the Wnt/β-catenin pathway and the commercially available PSC Cardiomyocyte Differentiation Kit. We underlined the need to optimize several parameters such as cell density or small molecule concentration (CHIR-99021, IWR-1) to obtain functional hiPSC-CMs. Both protocols yield a similar differentiation efficiency; therefore, the choice of a particular procedure may depend on the preferences of the experimenter.
摘要：
源自人类诱导的多能干细胞（hiPSC-CM）的心肌细胞提供了一个有吸引力的平台来评估心血管相关事件的机制以及开发和测试用于心脏病的新药。这项工作的重点是比较两种hiPSC-CM分化方案：基于Wnt/β-catenin途径的时间调制的GiWi方法和市售的PSC心肌细胞分化试剂盒。我们强调需要优化几个参数，如细胞密度或小分子浓度（CHIR-99021，IWR-1）以获得功能性hiPSC-CM。两种方案都产生相似的分化效率；因此，特定程序的选择可能取决于实验者的偏好。
