Abstract：
Organoids, three-dimensional, stem cell-based structures that mimic the cellular and functional architecture of tissues, have emerged as an innovative in vitro tool. They offer highly efficient models for studying both embryonic development and disease progression processes. Colon organoids can also be generated from biopsies obtained during a colonoscopy. However, the invasive nature of biopsy collection poses practical challenges and introduces biases when studying patients who are already afflicted. Therefore, the use of iPSC-derived colon organoids can be considered a more practical approach for researchers and patients alike. Numerous protocols have been published for generating colon organoids from iPSCs. While most of these protocols share a common developmental process, some are labor-intensive or require additional equipment. Taking these considerations into account, we present a cost-effective and straightforward yet functionally robust colon organoid protocol: (1) definitive endoderm differentiation, (2) hindgut endoderm differentiation, and (3) maturation of colon spheroids into mature organoids.
摘要：
类器官,三维，基于干细胞的结构，模拟组织的细胞和功能结构，已经成为一种创新的体外工具。它们为研究胚胎发育和疾病进展过程提供了高效的模型。结肠类器官也可以从结肠镜检查期间获得的活检产生。然而,活检采集的侵入性带来了实际挑战,并在研究已经患病的患者时引入了偏见.因此,对于研究人员和患者而言,使用iPSC衍生的结肠类器官可以被认为是一种更实用的方法.已经发表了许多用于从iPSC产生结肠类器官的方案。虽然大多数协议都有一个共同的发展过程，有些是劳动密集型的或需要额外的设备。考虑到这些因素，我们提出了一个具有成本效益和简单但功能稳健的结肠类器官方案：（1）定形内胚层分化，（2）后肠内胚层分化，和(3)结肠球状体成熟为成熟的类器官。
