PDF(Pubmed)
Abstract：
Bathymodioline mussels dominate deep-sea methane seep and hydrothermal vent habitats and obtain nutrients and energy primarily through chemosynthetic endosymbiotic bacteria in the bacteriocytes of their gill. However, the molecular mechanisms that orchestrate mussel host-symbiont interactions remain unclear. Here, we constructed a comprehensive cell atlas of the gill in the mussel Gigantidas platifrons from the South China Sea methane seeps (1100 m depth) using single-nucleus RNA-sequencing (snRNA-seq) and whole-mount in situ hybridisation. We identified 13 types of cells, including three previously unknown ones, and uncovered unknown tissue heterogeneity. Every cell type has a designated function in supporting the gill\'s structure and function, creating an optimal environment for chemosynthesis, and effectively acquiring nutrients from the endosymbiotic bacteria. Analysis of snRNA-seq of in situ transplanted mussels clearly showed the shifts in cell state in response to environmental oscillations. Our findings provide insight into the principles of host-symbiont interaction and the bivalves\' environmental adaption mechanisms.
摘要：
Bathymodioline贻贝在深海甲烷渗漏和热液喷口栖息地中占主导地位，并主要通过其g细菌细胞中的化学合成内共生细菌获得营养和能量。然而,协调贻贝宿主-共生体相互作用的分子机制仍不清楚。这里,我们使用单核RNA测序（snRNA-seq）和全装原位杂交，从南海甲烷渗漏（1100m深）的贻贝Gigantidasplatif中构建了g的全面细胞图谱。我们确定了13种类型的细胞，包括三个以前未知的，并发现未知的组织异质性。每个细胞类型都有一个指定的功能来支持g的结构和功能，为化学合成创造最佳环境，并有效地从内共生细菌中获取营养。对原位移植贻贝的snRNA-seq的分析清楚地表明了细胞状态响应于环境振荡的变化。我们的发现为宿主-共生体相互作用和双壳类动物的环境适应机制提供了见解。
