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Abstract：
Lonicera japonica Flos is a valuable herb in the Lonicerae family. While transcriptomic studies on L. japonica have focused on different tissues (stems, leaves, flowers) or flowering stages, few have investigated the molecular mechanisms underlying chemical composition synthesis influenced by exogenous factors, such as foliar fertilization. Moreover, most transcriptomic studies on L. Japonica have been conducted on chlorogenic acid and luteoloside, and the molecular synthesis mechanism of the overall chemical composition has not been analyzed. Methods: We conducted a single-factor, four-level foliar fertilization experiment using yeast polysaccharides. Different yeast polysaccharides concentrations were sprayed on L. japonica for six consecutive days with dynamic sampling. High-performance liquid chromatography determined the active ingredients in each group. The two groups exhibiting the most significant differences were selected for transcriptomic analysis to identify key synthetic genes responsible for L. japonica\'s active ingredients. Key results: Principal component analysis conducted on samples collected on September 8 revealed significant differences in the active ingredient amounts between the 0.1 g/L yeast polysaccharides treatment group and the control group. Transcriptome sequencing analysis identified 218 significantly differentially expressed genes, including 60 upregulated and 158 downregulated genes. Twelve differential genes involved in the chemical components synthesis pathway of L. japonica under yeast polysaccharides treatment were identified: PAL1, PAL2, PAL3, 4CL1, 4CL, CHS1, CHS2, CHS, CHI1, CHI2, F3H, and SOH. Conclusions: This study contributes to the theoretical understanding of essential synthetic genes associated with L. japonica\'s active ingredients. It offers data support for further gene exploration and sheds light on the molecular mechanisms underlying L. japonica quality formation. These findings hold significant implications for enhancing the content of secondary metabolites of L. japonica.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12298-024-01482-1.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12298-024-01482-1.
摘要：
金银花是金银花科中的一种有价值的草本植物。虽然对L.japonica的转录组学研究集中在不同的组织(茎，叶子,花）或开花阶段，很少有人研究了外源因素影响化学成分合成的分子机制，如叶面施肥。此外，大多数关于L.Japonica的转录组学研究都是在绿原酸和叶黄素上进行的，整体化学成分的分子合成机理尚未分析。方法：我们进行了单因素，使用酵母多糖进行四级叶面施肥实验。在动态采样的情况下，将不同浓度的酵母多糖连续6天喷雾在日本L.japonica上。高效液相色谱法测定各组中的活性成分。选择表现出最显着差异的两组进行转录组学分析，以鉴定负责日本L.japonica活性成分的关键合成基因。主要结果：对9月8日收集的样品进行的主成分分析显示，0.1g/L酵母多糖处理组与对照组之间的活性成分量存在显着差异。转录组测序分析确定了218个显著差异表达的基因，包括60个上调基因和158个下调基因。在酵母多糖处理下，鉴定了12个参与日本品系化学成分合成途径的差异基因：PAL1，PAL2，PAL3，4CL1，4CL，CHS1,CHS2,CHS,CHI1,CHI2,F3H,还有SOH.结论：本研究有助于从理论上理解与日本血吸虫活性成分相关的必需合成基因。它为进一步的基因探索提供了数据支持，并阐明了粳稻品质形成的分子机制。这些发现对提高日本血吸虫次生代谢产物的含量具有重要意义。
■在线版本包含补充材料，可在10.1007/s12298-024-01482-1获得。
■在线版本包含补充材料，可在10.1007/s12298-024-01482-1获得。
