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Abstract：
Ultrasound-assisted extraction (UAE) was evaluated as a green procedure to produce faba beans protein isolates from faba beans. Magnetic stirring was performed as conventional extraction. A three-level five-factor Box-Behnken Design (BBD) was applied to obtain the optimal UAE conditions to concurrently maximize extraction yield and protein content. The response surface methodology (RSM) showed a quadratic curvature for extraction yield and protein. The optimal extraction conditions were determined as: Power of 123 W, solute/solvent ratio of 0.06 (1:15 g/mL), sonication time of 41 min, and total volume of 623 mL with a desirability value of 0.82. Under these conditions, the extraction yield of 19. 75 ± 0.87 % (Protein yield of 67.84 %) and protein content of 92.87 ± 0.53 % were obtained for optimum ultrasound extraction. Control samples using magnetic stirring under similar conditions without ultrasound treatment showed an extraction yield of 16.41 ± 0.02 % (Protein yield of 54.65 %) and a protein content of 89. 88 ± 0.40 %. This shows that BBD can effectively be used to optimize the extraction of proteins from faba beans using optimal extraction conditions, resulting in a higher extraction yield and protein purity.
摘要：
超声辅助提取（UAE）被评估为从蚕豆生产蚕豆蛋白分离物的绿色程序。磁力搅拌作为常规提取进行。采用三级五因素Box-Behnken设计（BBD）来获得最佳的UAE条件，以同时最大化提取率和蛋白质含量。响应面方法（RSM）显示提取率和蛋白质的二次曲率。确定了最佳提取条件：功率为123W，溶质/溶剂比为0.06（1:15g/mL），超声处理时间41分钟，总体积为623mL，可取值为0.82。在这些条件下,提取率为19。对于最佳超声提取，获得75±0.87％（蛋白质产率为67.84％）和92.87±0.53％的蛋白质含量。在没有超声处理的类似条件下使用磁力搅拌的对照样品显示出16.41±0.02%的提取率(54.65%的蛋白质产率)和89的蛋白质含量。88±0.40%。这表明BBD可以有效地使用最佳提取条件来优化从蚕豆中提取蛋白质，导致更高的提取率和蛋白质纯度。
