Abstract：
Diabetes is a chronic disease with significant complications, necessitating regular treatment and checkups, which can be costly and time-consuming for patients. To address this, we developed the Sliding Microneedle (MN)-Lateral flow immunoassay strip (LFIAs) device that combines the advantages of MNs and LFIAs to detect IL-6, an independent biomarker for diabetes complications. This device offers rapid and highly sensitive detection of IL-6 by extracting interstitial fluid (ISF) through MNs and transferring it to LFIAs. The stainless MN, embedded in the 3D-printed Sliding MN-LFIAs device, was inserted into the skin at a 20° angle, minimizing blood contamination risk. With a filter paper attached to the MN surface, the device collected 4.65 ± 0.05 μL of ISF containing IL-6 within 90 s. The ISF was then transferred to the LFIAs using a running buffer. After a 15-min reaction, silver enhancement (SE) treatment was applied, allowing for the highly sensitive and specific detection of IL-6 at 102 pg/mL concentrations. The Sliding MN-LFIAs device successfully distinguished between normal and diabetic rat models, demonstrating its potential as an effective tool for detecting diabetes complications quickly and affordably.
摘要：
糖尿病是一种具有显著并发症的慢性疾病,需要定期治疗和检查，这对患者来说可能是昂贵且耗时的。为了解决这个问题，我们开发了滑动微针(MN)-侧流免疫分析试纸条(LFIA)装置,该装置结合了MNs和LFIA的优势来检测IL-6,这是糖尿病并发症的独立生物标志物.该装置通过MN提取间质液（ISF）并将其转移到LFIA，从而快速且高灵敏度地检测IL-6。不锈钢MN，嵌入3D打印的滑动MN-LFIA设备中，以20°的角度插入皮肤，减少血液污染的风险。MN表面贴着一张滤纸，装置在90秒内收集4.65±0.05μL含有IL-6的ISF。然后使用运行缓冲液将ISF转移至LFIA。反应15分钟后,应用银增强(SE)处理，允许在102pg/mL浓度下高度灵敏和特异性地检测IL-6。滑动MN-LFIA装置成功区分正常和糖尿病大鼠模型，证明其作为快速且经济地检测糖尿病并发症的有效工具的潜力。
