METHODS: Mice were randomly divided into control and chronic restraint stress (CRS) groups. The mice subjected to CRS were further divided into cognitive deficit (CRS-CD) and non-cognitive deficit (CRS-NCD) groups using hierarchical cluster analysis of novel object recognition test results. The composition and diversity of the gut microbiota were analyzed.
RESULTS: After being subjected to chronic restraint distress, the CRS-CD mice travelled shorter movement distances (p = 0.034 vs. CRS-NCD; p < 0.001 vs. control) and had a lower recognition index than the CRS-NCD (p < 0.0001 vs. CRS-NCD; p < 0.0001 vs. control) and control mice. The results revealed that 5 gut bacteria at genus levels were significantly different in the fecal samples of mice in the three groups. Further analyses demonstrated that Muricomes were not only significantly enriched in the CRS-CD group but also correlated with a decreased cognitive index. The area under the receiver operating curve of Muricomes for CRS-induced cognitive deficits was 0.96.
CONCLUSIONS: Our study indicates that the composition of the gut microbiota is involved in the development of cognitive deficits induced by chronic restraint stress. Further analysis revealed that Muricomes have the potential to predict the development of chronic stress-induced cognitive deficits in mice.
方法:小鼠随机分为对照组和慢性束缚应激(CRS)组。使用新物体识别测试结果的层次聚类分析,将接受CRS的小鼠进一步分为认知缺陷(CRS-CD)和非认知缺陷(CRS-NCD)组。分析了肠道菌群的组成和多样性。
结果:在遭受慢性束缚困扰后,CRS-CD小鼠运动距离较短(p=0.034vs.CRS-NCD;p<0.001vs.对照),识别指数低于CRS-NCD(p<0.0001vs.CRS-NCD;p<0.0001vs.对照)和对照小鼠。结果表明,三组小鼠粪便样本中5种肠道菌属水平差异显著。进一步的分析表明,Muricome不仅在CRS-CD组中显着丰富,而且与认知指数下降相关。CRS诱导的认知缺陷的Muricome的接受者工作曲线下面积为0.96。
结论:我们的研究表明,肠道菌群的组成与慢性束缚应激引起的认知缺陷的发展有关。进一步的分析显示,Muricome有可能预测小鼠慢性应激诱导的认知缺陷的发展。