Abstract：
The phytoestrogens daidzein and genistein are ubiquitous in human food. This study aimed to elucidate their anxiety-liked effects, their effects on the reproductive organs, and the molecular mechanism behind any anxiety-liked effects in intact adult male Wistar rats. These phytoestrogens are of interest due to their posited health benefits, particularly for female, but with some effect on males as well. This study comprised two experiments: (1) Male Wistar rats received either a vehicle, daidzein, or genistein (0.25, 0.50, or 1.00 mg/kg) by subcutaneously injection for four weeks. They were then tested for anxiety-liked behaviors. Then, the brain monoamines in anxiolytic rats were determined; (2) The modulation of gamma aminobutyric acid receptors by phytoestrogens was further analyzed by administration of diazepam to phytoestrogen-treated rats before behavioral tests. In the first experiment, the biological parameters measured, including body weight, daily food intake and reproductive organ weights were unaffected by either genistein or daidzein. However, anxiolytic-like effect was observed in the low-dose daidzein (0.25 mg/kg) group. Higher doses of daidzein or genistein of all doses had no effect. Further, the low-dose daidzein did not alter brain monoamine levels. In the second experiment, the anxiolytic-like behavior of daidzein-treated rats receiving diazepam did not differ from that of the rats treated with just diazepam or just daidzein. In conclusion, 4-week exposure to daidzein or genistein had no negative effects on the reproductive organs, body weight, food intake, anxiogenic-like behavior, or monoaminergic and diazepam-modulated GABAergic neurotransmissions of intact male rats. However, beneficial anxiolytic-like effects were apparent after low-dose treatment with daidzein.
摘要：
植物雌激素大豆苷元和染料木素在人类食物中普遍存在。这项研究旨在阐明他们的焦虑效应，它们对生殖器官的影响，以及完整的成年雄性Wistar大鼠中任何焦虑样效应背后的分子机制。这些植物雌激素由于其假定的健康益处而受到关注，尤其是女性,但对男性也有一些影响。这项研究包括两个实验：（1）雄性Wistar大鼠接受了一种载体，Daidzein,或染料木素（0.25、0.50或1.00mg/kg）皮下注射4周。然后对他们进行了类似焦虑的行为测试。然后，测定了抗焦虑大鼠的脑单胺；（2）在行为测试之前，通过向植物雌激素治疗的大鼠施用地西泮进一步分析了植物雌激素对γ氨基丁酸受体的调节。在第一个实验中,测量的生物参数，包括体重,每天的食物摄入量和生殖器官重量不受染料木素或大豆黄酮的影响。然而,在低剂量大豆苷元（0.25mg/kg）组中观察到抗焦虑作用。所有剂量的较高剂量的大豆苷元或染料木素没有作用。Further,低剂量的大豆苷元不会改变大脑中的单胺水平.在第二个实验中,接受地西泮的大豆苷元治疗的大鼠的抗焦虑行为与仅接受地西泮或大豆苷元治疗的大鼠的抗焦虑行为没有差异。总之,4周暴露于大豆黄酮或染料木素对生殖器官没有负面影响，体重,食物摄入量,类似焦虑的行为，或完整雄性大鼠的单胺能和地西泮调节的GABA能神经传递。然而,用大豆苷元低剂量治疗后，有益的抗焦虑作用明显。
