Abstract：
The health and productivity of broilers may be improved by optimizing the availability and levels of trace minerals (TM) in their feed, especially in the presence of parasites. This study investigated the effects of replacing inorganic TM (ITM) with an advanced chelate technology-based 7 TM (ACTM) on performance, hematology, lesion score, oocyst shedding, gut morphology, and tight junction structure in broilers challenged with mixed Eimeria species. There were 480 1-day-old broiler chickens divided into 5 groups: uninfected negative control and recommended levels of ITM (NC); infected positive control and recommended levels of ITM (PC); or PC supplemented with salinomycin (SAL); PC diet with 50 % ACTM instead of ITM (ACTM50); or PC diet with 100 % ACTM instead of ITM (ACTM100). All groups, except NC, were orally challenged with mixed Eimeria spp. oocysts on day 14. Each group had 6 replicate cages, with 16 birds per replicate. The results showed that the NC, SAL, and ACTM100 groups had higher (P < 0.05) body weight, average daily gain (ADG), and European production efficiency index (EPEI), as well as a lower (P < 0.05) feed conversion, mortality rate, and heterophile to lymphocyte ratio compared to the PC group, with the NC group having the highest ADG and EPEI throughout the experiment. The SAL and ACTM100 groups had lower (P < 0.05) intestinal lesion scores and oocyst numbers compared to the PC group, although all coccidiosis-challenged groups had higher oocyst shedding compared to the NC group. On day 24, the challenged birds in the SAL and ACTM100 groups had higher (P < 0.05) villus height and surface area in the duodenum and ileum, as well as a higher (P < 0.05) villus height to crypt depth ratio in the jejunum. The expression levels of jejunal CLDN1 and ZO-1 were also higher (P < 0.05) in the ACTM100 and SAL groups compared to the PC and ACTM50 groups at 24 days of age. In conclusion, while using ACTM in broiler diets at 50 % of the commercial recommended levels maintained performance and physiological responses, complete replacement with ACTM improved growth performance and intestinal health characteristics, similar to salinomycin under Eimeria challenge conditions.
摘要：
通过优化饲料中微量矿物质（TM）的可用性和水平，可以改善肉鸡的健康和生产力。尤其是在有寄生虫的情况下.这项研究调查了用基于高级螯合物技术的7TM（ACTM）代替无机TM（ITM）对性能的影响，血液学,病变评分,卵囊脱落,肠道形态学,以及混合艾美球虫物种挑战的肉鸡的紧密连接结构。有480只1日龄肉鸡，分为5组：未感染的阴性对照和推荐的ITM水平（NC）；感染的阳性对照和推荐的ITM水平（PC）；或补充盐霉素（SAL）的PC；含50％ACTM代替ITM的PC饮食（ACTM50）；或含100％ACTM代替ITM的PC饮食（ACTM100）。所有团体,除了NC,用混合艾美球虫进行口头攻击。第14天的卵囊。每组有6个重复的笼子，每次复制16只鸟。结果表明,NC,SAL,ACTM100组体重较高(P<0.05),平均日增益（ADG），和欧洲生产效率指数(EPEI)，以及较低的饲料转化率(P<0.05)，死亡率,与PC组相比，异源性淋巴细胞与淋巴细胞的比率，在整个实验中，NC组的ADG和EPEI最高。与PC组相比，SAL和ACTM100组肠病变评分和卵囊数量较低（P<0.05），尽管与NC组相比，所有球虫病攻击组的卵囊脱落率都较高。在第24天，SAL和ACTM100组攻击的鸟类十二指肠和回肠的绒毛高度和表面积较高（P<0.05），空肠绒毛高度与隐窝深度比较高（P<0.05）。在24日龄时，ACTM100和SAL组的空肠CLDN1和ZO-1的表达水平也高于PC和ACTM50组（P<0.05）。总之,虽然在50％的商业推荐水平的肉鸡饮食中使用ACTM保持了性能和生理反应，完全替代ACTM改善了生长性能和肠道健康特征，在艾美球虫攻击条件下与盐霉素相似。
