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Abstract：
Retinal progenitor cells (RPCs) are a multipotent and highly proliferative population that give rise to all retinal cell types during organogenesis. Defining their molecular signature is a key step towards identifying suitable approaches to treat visual impairments. Here, we performed RNA-sequencing of whole eyes from Xenopus at three embryonic stages and used differential expression analysis to define the transcriptomic profiles of optic tissues containing proliferating and differentiating RPCs during retinogenesis. Gene Ontology and KEGG pathway analyses showed that genes associated with developmental pathways (including Wnt and Hedgehog signaling) were upregulated during the period of active RPC proliferation in early retinal development (Nieuwkoop Faber st. 24 and 27). Developing eyes had dynamic expression profiles and shifted to enrichment for metabolic processes and phototransduction during RPC progeny specification and differentiation (st. 35). Furthermore, conserved adult eye regeneration genes were also expressed during early retinal development including sox2, pax6, nrl, and Notch signaling components. The eye transcriptomic profiles presented here span RPC proliferation to retinogenesis and included regrowth-competent stages. Thus, our dataset provides a rich resource to uncover molecular regulators of RPC activity and will allow future studies to address regulators of RPC proliferation during eye repair and regrowth.
摘要：
视网膜祖细胞（RPC）是一种多能且高度增殖的群体，在器官发生过程中会产生所有视网膜细胞类型。定义它们的分子特征是确定治疗视觉障碍的合适方法的关键步骤。这里,我们在3个胚胎阶段对非洲爪的全眼进行了RNA测序,并使用差异表达分析来确定视网膜发生过程中含有增殖和分化RPCs的视组织的转录组学谱.基因本体论和KEGG途径分析表明，在视网膜早期发育的主动RPC增殖期间，与发育途径相关的基因（包括Wnt和Hedgehog信号）被上调（NieuwkoopFaberst。24和27）。发育中的眼睛具有动态表达谱，并在RPC后代规范和分化过程中转向富集代谢过程和光转导（st.35).此外，保守的成人眼再生基因也在早期视网膜发育过程中表达，包括sox2，pax6,nrl,和陷波信号组件。此处提供的眼睛转录组概况涵盖了RPC增殖到视网膜发生，并包括再生能力阶段。因此，我们的数据集为揭示RPC活性的分子调节因子提供了丰富的资源,并将允许未来的研究解决眼修复和再生过程中RPC增殖的调节因子.
