Abstract：
METHODS: This study aims to identify the gut enterotypes that explain differential responses to intervention with whole grain rye by proposing an \"enterotype - metabolic\" model.
RESULTS: A 12-week randomized controlled trial is conducted in Chinese adults, with 79 subjects consuming whole grain products with fermented rye bran (FRB) and 77 consuming refined wheat products in this exploratory post-hoc analysis. Responders or non-responders are identified according to whether blood glucose decreased by more than 10% after rye intervention. Compared to non-responders, responders in FRB have higher baseline Bacteroides (p < 0.001), associated with reduced blood glucose (p < 0.001), increased Faecalibacterium (p = 0.020) and Erysipelotrichaceae_UCG.003 (p = 0.022), as well as deceased 7β-hydroxysteroid dehydrogenase (p = 0.033) after intervention. The differentiated gut microbiota and metabolites between responders and non-responders after intervention are enriched in aminoacyl-tRNA biosynthesis.
CONCLUSIONS: The work confirms the previously suggested importance of microbial enterotypes in differential responses to whole grain interventions and supports taking enterotypes into consideration for improved efficacy of whole grain intervention for preventing type 2 diabetes. Altered short-chain fatty acids and bile acid metabolism might be a potential mediator for the beneficial effects of whole grain rye on glucose metabolism.
RESULTS: A 12-week randomized controlled trial is conducted in Chinese adults, with 79 subjects consuming whole grain products with fermented rye bran (FRB) and 77 consuming refined wheat products in this exploratory post-hoc analysis. Responders or non-responders are identified according to whether blood glucose decreased by more than 10% after rye intervention. Compared to non-responders, responders in FRB have higher baseline Bacteroides (p < 0.001), associated with reduced blood glucose (p < 0.001), increased Faecalibacterium (p = 0.020) and Erysipelotrichaceae_UCG.003 (p = 0.022), as well as deceased 7β-hydroxysteroid dehydrogenase (p = 0.033) after intervention. The differentiated gut microbiota and metabolites between responders and non-responders after intervention are enriched in aminoacyl-tRNA biosynthesis.
CONCLUSIONS: The work confirms the previously suggested importance of microbial enterotypes in differential responses to whole grain interventions and supports taking enterotypes into consideration for improved efficacy of whole grain intervention for preventing type 2 diabetes. Altered short-chain fatty acids and bile acid metabolism might be a potential mediator for the beneficial effects of whole grain rye on glucose metabolism.
摘要：
方法：本研究旨在通过提出“肠型-代谢”模型来确定解释对全粒黑麦干预的差异反应的肠道肠型。
结果：一项为期12周的随机对照试验在中国成年人中进行，在这种探索性事后分析中，有79名受试者食用带有发酵黑麦麸皮（FRB）的全谷物产品，而77名受试者食用精制小麦产品。根据黑麦干预后血糖是否降低超过10%来鉴定应答者或非应答者。与非响应者相比，FRB中的反应者具有较高的基线拟杆菌（p<0.001），与血糖降低相关（p<0.001），增加的粪杆菌（p=0.020）和赤毒菌科_UCG.003（p=0.022），以及干预后死亡的7β-羟基类固醇脱氢酶（p=0.033）。干预后应答者和非应答者之间分化的肠道微生物群和代谢物富集在氨酰基-tRNA生物合成中。
结论：这项工作证实了先前提出的微生物肠型在对全谷物干预的不同反应中的重要性，并支持将肠型纳入考虑，以提高全谷物干预预防2型糖尿病的功效。短链脂肪酸和胆汁酸代谢的改变可能是全谷物黑麦对葡萄糖代谢有益作用的潜在介质。
结果：一项为期12周的随机对照试验在中国成年人中进行，在这种探索性事后分析中，有79名受试者食用带有发酵黑麦麸皮（FRB）的全谷物产品，而77名受试者食用精制小麦产品。根据黑麦干预后血糖是否降低超过10%来鉴定应答者或非应答者。与非响应者相比，FRB中的反应者具有较高的基线拟杆菌（p<0.001），与血糖降低相关（p<0.001），增加的粪杆菌（p=0.020）和赤毒菌科_UCG.003（p=0.022），以及干预后死亡的7β-羟基类固醇脱氢酶（p=0.033）。干预后应答者和非应答者之间分化的肠道微生物群和代谢物富集在氨酰基-tRNA生物合成中。
结论：这项工作证实了先前提出的微生物肠型在对全谷物干预的不同反应中的重要性，并支持将肠型纳入考虑，以提高全谷物干预预防2型糖尿病的功效。短链脂肪酸和胆汁酸代谢的改变可能是全谷物黑麦对葡萄糖代谢有益作用的潜在介质。
