Abstract：
The influence of non-opsonized and opsonized S. aureus 2879M and E. coli 321 strains on the total strength of interaction between the endothelial cell and neutrophil during the docking process was studied using in vitro model of experimental septicemia. We observed a decrease in the force and work of adhesion between receptors of neutrophils and endothelial cells under the influence of non-opsonized strains and further decrease in the affinity of single interactions between cells under the influence of opsonized S. aureus, which was compensated by an increase in the number of contacts, as well as an increase in the force of adhesion under the influence of opsonized E. coli compared to non-opsonized bacteria, which remained below the control level, while adhesion work reaches the control level. Thus, opsonization of S. aureus aggravates the \"immunological uncoupling\" between neutrophils and endothelial cells, while opsonization of E. coli reduces the pathological effect compared to non-opsonized bacteria.
摘要：
使用实验性败血症的体外模型研究了未调理和调理的金黄色葡萄球菌2879M和大肠杆菌321菌株对对接过程中内皮细胞和中性粒细胞之间相互作用的总强度的影响。我们观察到在非调理菌株的影响下，嗜中性粒细胞受体和内皮细胞之间的粘附力和功降低，并且在调理金黄色葡萄球菌的影响下，细胞之间的单个相互作用的亲和力进一步降低，这通过增加接触数量来补偿，以及与未调理细菌相比，在调理大肠杆菌的影响下粘附力的增加，仍然低于控制水平，而粘合工作达到控制水平。因此，金黄色葡萄球菌的调理作用加重了中性粒细胞和内皮细胞之间的“免疫解偶联”，而与未调理的细菌相比，大肠杆菌的调理作用降低了病理效应。
