PDF(Pubmed)
Abstract：
Type II crustacean hyperglycemic hormone (CHH) neuropeptides play diverse roles in crustaceans. In the hermaphrodite shrimp Lysmata vittata, two transcripts of type II CHHs (molt-inhibiting hormone/gonad-inhibiting hormone, MIH/GIH1 and MIH/GIH2) were identified by transcriptome sequencing, and MIH/GIH1 was later named Lvit-GIH1 for its inhibitory effect on ovarian development. Based on the high similarity of MIH/GIH2 to Lvit-GIH1, we named tentatively MIH/GIH2 as Lvit-GIH2 and explored the role of Lvit-GIH2 in ovarian development. The open reading frame (ORF) of Lvit-GIH2 was 333 bp in length, encoding a precursor consisted of a 32-aa signal peptide and a 78-aa mature peptide, which shared high sequence similarity with the type II subfamily peptides in crustaceans. Notably, Lvit-GIH2 was widely expressed in multiple tissues. The qRT-PCR findings indicated a rising trend in the expression of Lvit-GIH2 from the male phase to the euhermaphrodite phase. Both RNA interference and addition of GIH2 recombinant proteins (rGIH2) experiments showed that Lvit-GIH2 suppressed Lvit-Vg expression in hepatopancreas and Lvit-VgR expression in ovary. To further investigate the role of Lvit-GIH2 in ovarian development, the RNA-sequence analysis was performed to examine the changes in ovary after addition of rGIH2. The results showed that the pathways (Cysteine and methionine metabolism, Apoptosis-multiple species, etc.) and the genes (17bHSD8, IGFR, CHH, etc.) related to ovarian development were negatively regulated by rGIH2. In brief, Lvit-GIH2 might inhibit the ovarian development in L. vittata.
摘要：
II型甲壳类高血糖激素（CHH）神经肽在甲壳类动物中起着不同的作用。在雌雄同体的虾Lysmatavittata中，II型CHHs（蜕皮抑制激素/性腺抑制激素，MIH/GIH1和MIH/GIH2）通过转录组测序鉴定，MIH/GIH1因其对卵巢发育的抑制作用而被命名为Lvit-GIH1。基于MIH/GIH2与Lvit-GIH1的高度相似性，我们暂时将MIH/GIH2命名为Lvit-GIH2，并探讨了Lvit-GIH2在卵巢发育中的作用。Lvit-GIH2的开放阅读框(ORF)长度为333bp,编码由32-aa信号肽和78-aa成熟肽组成的前体，与甲壳类动物的II型亚家族肽具有高度的序列相似性。值得注意的是,Lvit-GIH2在多种组织中广泛表达。qRT-PCR结果表明，Lvit-GIH2的表达从雄性期到雌雄同体期呈上升趋势。RNA干扰和添加GIH2重组蛋白（rGIH2）实验均显示Lvit-GIH2抑制肝胰腺中的Lvit-Vg表达和卵巢中的Lvit-VgR表达。为了进一步研究Lvit-GIH2在卵巢发育中的作用，RNA序列分析用于检测添加rGIH2后卵巢的变化.结果表明，这些途径(半胱氨酸和蛋氨酸代谢，凋亡-多种物种，等。)和基因(17bHSD8，IGFR，CHH,等。）与卵巢发育有关的rGIH2负调控。简而言之,Lvit-GIH2可能抑制维塔塔乳杆菌的卵巢发育。
