METHODS: This work develops polyacrylamide and alginate-based synthetic clots that are compatible with particle image velocimetry (PIV) and radiographic imaging techniques while maintaining mechanical properties of \'soft\' and \'hard\' human clots. Dynamic mechanical analysis testing using an HR2-Rheometer demonstrates comparable mechanical properties to human clots previously tested by this research group and provided in existing literature.
RESULTS: The synthetic clots are formulated with either 0.5% w/v polyethylene microspheres for PIV visualization or 20% w/v barium sulfate for angiographic visualization, enabling real-time imaging of clot behavior during thrombectomy simulations. The soft formulation shows compressive and shear properties of ~12 kPa and 2-3 kPa, respectively. The hard clots are 3-4 times stiffer, with compressive and shear properties of 41-42 kPa and 8-9 kPa, respectively.
CONCLUSIONS: Standardized synthetic clots offer a platform for reproducible device testing. This provides a greater understanding of mechanical thrombectomy device efficacy, which may lead to quantifiable advances in device development and eventual improved clinical outcomes.
方法:这项工作开发了基于聚丙烯酰胺和藻酸盐的合成凝块,这些凝块与粒子图像测速(PIV)和射线照相成像技术兼容,同时保持了“软”和“硬”的机械性能。使用HR2-流变仪的动态机械分析测试证明了与该研究小组先前测试并在现有文献中提供的人类凝块相当的机械性能。
结果:合成凝块用0.5%w/v聚乙烯微球配制用于PIV可视化,或用20%w/v硫酸钡配制用于血管造影可视化。在血栓切除术模拟过程中,能够实时成像凝块行为。软配方显示〜12kPa和2-3kPa的压缩和剪切性能,分别。硬凝块硬3-4倍,具有41-42kPa和8-9kPa的压缩和剪切性能,分别。
结论:标准化合成凝块为可重复的设备测试提供了平台。这提供了对机械取栓装置功效的更多了解,这可能导致器械开发的可量化进展和最终改善的临床结果。