Abstract：
OBJECTIVE: To determine if host urinary biomarker profiles could distinguish between tubercular uveitis (TBU) and other uveitic diseases (OUD) in patients with and without HIV infection.
METHODS: Concentrations of 29 different host biomarkers were measured in urine samples using the Luminex platform. Data were analyzed to describe differences between patients diagnosed with and without TBU and with and without HIV co-infection.
RESULTS: One-hundred-and-eighteen urine samples were collected and 39% participants were diagnosed as TBU+. Mean age TBU+ was 39.3±13.6 years with 45.7% males. Anterior and panuveitis and unilateral involvement were most common. 32.6% were TBU+HIV+ (median CD4+=215) while 40.2% were OUD+HIV+ (median CD4+=234). Only sVEGF3 was decreased in TBU+ versus OUD+ (p=0.03), regardless of HIV status. Some biomarkers were significantly raised in HIV+ TBU+ compared to HIV- TBU+: sIL-6Rα, CD30, sRAGE , sTNFR I&-II, IP-10, MIP-1β, sEGFR and Ferritin. HIV+ OUD+ had increased sVEGFR3, CD30, sIL-6Rα, IP-10, sTNFR I&-II, Ferritin and Haptoglobin compared to HIV- OUD+. VEGF-A (p = 0.04) was decreased in HIV+ OUD+ versus HIV- OUD+.
CONCLUSIONS: Decreased urinary concentrations of VEGFR3 were observed in TBU+ compared to TBU-. HIV+ individuals demonstrated increased concentrations of multiple urinary analytes when compared to HIV- patients with uveitis.
METHODS: Concentrations of 29 different host biomarkers were measured in urine samples using the Luminex platform. Data were analyzed to describe differences between patients diagnosed with and without TBU and with and without HIV co-infection.
RESULTS: One-hundred-and-eighteen urine samples were collected and 39% participants were diagnosed as TBU+. Mean age TBU+ was 39.3±13.6 years with 45.7% males. Anterior and panuveitis and unilateral involvement were most common. 32.6% were TBU+HIV+ (median CD4+=215) while 40.2% were OUD+HIV+ (median CD4+=234). Only sVEGF3 was decreased in TBU+ versus OUD+ (p=0.03), regardless of HIV status. Some biomarkers were significantly raised in HIV+ TBU+ compared to HIV- TBU+: sIL-6Rα, CD30, sRAGE , sTNFR I&-II, IP-10, MIP-1β, sEGFR and Ferritin. HIV+ OUD+ had increased sVEGFR3, CD30, sIL-6Rα, IP-10, sTNFR I&-II, Ferritin and Haptoglobin compared to HIV- OUD+. VEGF-A (p = 0.04) was decreased in HIV+ OUD+ versus HIV- OUD+.
CONCLUSIONS: Decreased urinary concentrations of VEGFR3 were observed in TBU+ compared to TBU-. HIV+ individuals demonstrated increased concentrations of multiple urinary analytes when compared to HIV- patients with uveitis.
摘要：
目的：确定宿主尿液生物标志物谱是否可以区分有和没有HIV感染的患者的结核性葡萄膜炎（TBU）和其他葡萄膜疾病（OUD）。
方法：使用Luminex平台在尿液样品中测量29种不同宿主生物标志物的浓度。分析数据以描述诊断有和没有TBU以及有和没有HIV共感染的患者之间的差异。
结果：收集了118份尿液样本，39%的参与者被诊断为TBU+。TBU+的平均年龄为39.3±13.6岁，男性占45.7%。前和全葡萄膜炎和单侧受累最常见。32.6%为TBU+HIV+(CD4+中位数=215),40.2%为OUD+HIV+(CD4+中位数=234)。只有sVEGF3在TBU+与OUD+中降低（p=0.03），无论艾滋病毒状况如何。与HIV-TBU+相比，HIV+TBU+中的一些生物标志物显著升高：sIL-6Rα，CD30,sRAGE,sTNFRI&-II,IP-10,MIP-1β,sEGFR和铁蛋白。HIV+OUD+有增加的sVEGFR3、CD30、sIL-6Rα、IP-10,sTNFRI&-II,铁蛋白和结合珠蛋白与HIV-OUD+的比较。与HIV-OUD+相比,VEGF-A(p=0.04)在HIV+OUD+中降低。
结论：与TBU-相比，在TBU+中观察到VEGFR3的尿浓度降低。当与患有葡萄膜炎的HIV患者相比时,HIV+个体表现出增加的多种尿分析物浓度。
方法：使用Luminex平台在尿液样品中测量29种不同宿主生物标志物的浓度。分析数据以描述诊断有和没有TBU以及有和没有HIV共感染的患者之间的差异。
结果：收集了118份尿液样本，39%的参与者被诊断为TBU+。TBU+的平均年龄为39.3±13.6岁，男性占45.7%。前和全葡萄膜炎和单侧受累最常见。32.6%为TBU+HIV+(CD4+中位数=215),40.2%为OUD+HIV+(CD4+中位数=234)。只有sVEGF3在TBU+与OUD+中降低（p=0.03），无论艾滋病毒状况如何。与HIV-TBU+相比，HIV+TBU+中的一些生物标志物显著升高：sIL-6Rα，CD30,sRAGE,sTNFRI&-II,IP-10,MIP-1β,sEGFR和铁蛋白。HIV+OUD+有增加的sVEGFR3、CD30、sIL-6Rα、IP-10,sTNFRI&-II,铁蛋白和结合珠蛋白与HIV-OUD+的比较。与HIV-OUD+相比,VEGF-A(p=0.04)在HIV+OUD+中降低。
结论：与TBU-相比，在TBU+中观察到VEGFR3的尿浓度降低。当与患有葡萄膜炎的HIV患者相比时,HIV+个体表现出增加的多种尿分析物浓度。
