PDF(Pubmed)
Abstract：
OBJECTIVE: As one of the main etiologic agents of infectious diseases in pigs, pseudorabies virus (PRV) infections have caused enormous economic losses worldwide. EP0, one of the PRV early proteins (EP) plays a vital role in PRV infections, but the mechanisms are unclear.
OBJECTIVE: This study examined the function of EP0 to provide a direction for its in-depth analysis.
METHODS: In this study, the EP0-deleted PRV mutant was obtained, and Tandem Mass Tag-based proteomic analysis was used to screen the differentially expressed proteins (DEPs) quantitatively in EP0-deleted PRV- or wild-type PRV-infected porcine kidney 15 cells.
RESULTS: This study identified 7,391 DEPs, including 120 and 21 up-regulated and down-regulated DEPs, respectively. Western blot analysis confirmed the changes in the expression of the selected proteins, such as speckled protein 100. Comprehensive analysis revealed 141 DEPs involved in various biological processes and molecular functions, such as transcription regulator activity, biological regulation, and localization.
CONCLUSIONS: These results holistically outlined the functions of EP0 during a PRV infection and might provide a direction for more detailed function studies of EP0 and the stimulation of lytic PRV infections.
OBJECTIVE: This study examined the function of EP0 to provide a direction for its in-depth analysis.
METHODS: In this study, the EP0-deleted PRV mutant was obtained, and Tandem Mass Tag-based proteomic analysis was used to screen the differentially expressed proteins (DEPs) quantitatively in EP0-deleted PRV- or wild-type PRV-infected porcine kidney 15 cells.
RESULTS: This study identified 7,391 DEPs, including 120 and 21 up-regulated and down-regulated DEPs, respectively. Western blot analysis confirmed the changes in the expression of the selected proteins, such as speckled protein 100. Comprehensive analysis revealed 141 DEPs involved in various biological processes and molecular functions, such as transcription regulator activity, biological regulation, and localization.
CONCLUSIONS: These results holistically outlined the functions of EP0 during a PRV infection and might provide a direction for more detailed function studies of EP0 and the stimulation of lytic PRV infections.
摘要：
目的:作为猪感染性疾病的主要病原之一,伪狂犬病病毒（PRV）感染在全球范围内造成了巨大的经济损失。EP0是PRV早期蛋白之一（EP），在PRV感染中起着至关重要的作用，但机制尚不清楚。
目的：本研究检查了EP0的功能，为其深入分析提供了方向。
方法：在本研究中，获得了EP0缺失的PRV突变体，和基于串联质量标签的蛋白质组分析用于定量筛选EP0缺失的PRV或野生型PRV感染的猪肾细胞中的差异表达蛋白(DEP)。
结果：这项研究确定了7,391个DEP，包括120和21个上调和下调的DEP，分别。蛋白质印迹分析证实了所选蛋白质表达的变化，如斑点蛋白100。综合分析显示141个DEP参与各种生物过程和分子功能，如转录调节活性，生物调节，和本地化。
结论：这些结果全面概述了EP0在PRV感染期间的功能，可能为更详细的EP0功能研究和溶解性PRV感染的刺激提供了方向。
目的：本研究检查了EP0的功能，为其深入分析提供了方向。
方法：在本研究中，获得了EP0缺失的PRV突变体，和基于串联质量标签的蛋白质组分析用于定量筛选EP0缺失的PRV或野生型PRV感染的猪肾细胞中的差异表达蛋白(DEP)。
结果：这项研究确定了7,391个DEP，包括120和21个上调和下调的DEP，分别。蛋白质印迹分析证实了所选蛋白质表达的变化，如斑点蛋白100。综合分析显示141个DEP参与各种生物过程和分子功能，如转录调节活性，生物调节，和本地化。
结论：这些结果全面概述了EP0在PRV感染期间的功能，可能为更详细的EP0功能研究和溶解性PRV感染的刺激提供了方向。
