Abstract：
UNASSIGNED: Background. In diagnosing insect venom allergy and making immunotherapy decisions, clinical history, skin tests, and specific serum IgE levels are commonly utilized. This study aims to emphasize the clinical significance of using the basophil activation test in accurately identifying sensitivities in individuals with insect venom allergy and to compare its effectiveness with other testing methods. Methods. This study included a total of 43 patients, who experienced at least one systemic allergic reaction following insect stings and were deemed suitable for immunotherapy.Basophil activation test, specific serum IgE levels, and skin prick test results utilized in making immunotherapy treatment decisions were recorded. Results. Our study determined that the overall clinical sensitivities of the basophil activation test (BAT), specific serum IgE (spIgE), and skin prick test (SPT) for apis mellifera were 95.5%, 95.7%, and 48.4% respectively, while for vespula vulgaris, they were 83.3%, 100%, and 33.3%. Based on these results, the prediction of systemic reactions to bee stings is ordered as spIgE > BAT > SPT. Additionally, early-stage skin prick tests showed a sensitivity of 67% and specificity of 50% at a cut-off value of 1.5 mm, and 33% sensitivity and 83% specificity at 2.5 mm. Conclusions. This study demonstrates that the basophil activation test (BAT) can provide a high positive predictive value in immunotherapy treatment decisions and offer significant insights in clinical practices.
摘要：
■背景。在诊断昆虫毒液过敏和做出免疫治疗决定时，临床病史,皮肤试验,和特异性血清IgE水平通常被利用。这项研究旨在强调使用嗜碱性粒细胞激活测试在准确识别昆虫毒液过敏个体的敏感性方面的临床意义，并将其与其他测试方法的有效性进行比较。方法。这项研究共纳入43名患者，在昆虫叮咬后至少经历了一次全身过敏反应，被认为适合免疫疗法。嗜碱性粒细胞激活试验,特异性血清IgE水平，并记录了用于制定免疫治疗治疗决策的皮肤点刺试验结果.结果。我们的研究确定了嗜碱性粒细胞激活试验（BAT）的总体临床敏感性，特异性血清IgE（spIgE），刺槐的皮肤点刺试验（SPT）为95.5％，95.7%,和48.4%，而对于vespulgaris，占83.3%,100%,和33.3%。基于这些结果,对蜜蜂叮咬的系统反应的预测顺序为spIgE>BAT>SPT。此外,早期皮肤点刺试验显示，在1.5毫米的临界值下，敏感性为67%，特异性为50%，在2.5mm时，灵敏度为33%，特异性为83%。Conclusions.这项研究表明，嗜碱性粒细胞活化试验（BAT）可以在免疫治疗治疗决策中提供高阳性预测值，并在临床实践中提供重要见解。
