METHODS: In this study, we analyzed full-length STLV-1 genome sequences obtained from 5 independent troops including a total of 68 JMs.
RESULTS: The overall nucleotide heterogeneity was 4.7%, and the heterogeneity among the troops was 2.1%, irrespective of the formation of distinct subclusters in each troop. Moreover, the heterogeneity within each troop was extremely low (>99% genome homology) compared with cases of STLV-1 in African non-human primates as well as humans. It was previously reported that frequent G-to-A single-nucleotide variants (SNVs) occur in HTLV-1 proviral genomes in both ATL patients and HTLV-1 carriers, and that a G-to-A hypermutation is associated with the cellular antiviral restriction factor, Apobec3G. Surprisingly, these SNVs were scarcely observed in the STLV-1 genomes in JMs.
CONCLUSIONS: Taken together, these results indicate that STLV-1 genomes in JMs are highly homologous, at least in part due to the lack of Apobec3G-dependent G-to-A hypermutation.
方法:在本研究中,我们分析了从5个独立部队获得的全长STLV-1基因组序列,包括总共68个JMs。
结果:总体核苷酸异质性为4.7%,部队之间的异质性是2.1%,无论在每个部队中形成不同的子组。此外,与非洲非人灵长类动物和人类的STLV-1病例相比,每个部队内部的异质性极低(基因组同源性>99%).先前有报道称,ATL患者和HTLV-1携带者的HTLV-1前病毒基因组中会出现频繁的G-to-A单核苷酸变异(SNV),并且G到A的超突变与细胞抗病毒限制因子有关,Apobe3G.令人惊讶的是,这些SNV在JMs的STLV-1基因组中几乎没有观察到。
结论:综合起来,这些结果表明JMs中的STLV-1基因组是高度同源的,至少部分是由于缺乏Apobe3G依赖性G-to-A超突变。