PDF(Pubmed)
Abstract：
The environmental contamination by extremophile Aspergillus species, i.e., Aflatoxin B1, is hardly controllable in Southeast Asia and Sub-Saharan Africa, which lack handling resources and controlled storage facilities. Acute aflatoxicosis poisoning from aflatoxin-prone dietary staples could cause acute hepatic necrosis, acute liver failure, and death. Here, as the cheaper, more straightforward, and facile on-site diagnostic kit is needed, we report an ultraviolet-excitable optical aptasensor based on a fluorinated ethylene propylene film strip. Molecular dynamics on the aptamer.AFB1 complex revealed that the AFB1 to the aptamer increases the overall structural stability, suggesting that the aptamer design is suitable for the intended application. Under various influencing factors, the proposed label-free strategy offers a fast 20-min on-site fabrication simplicity and 19-day shelf-life. The one-pot incubation provides an alternative to catalytic detection and exhibited 4 times reusability. The recovery of crude brown sugar, processed peanuts, and long-grain rice were 102.74 ± 0.41 (n = 3), 86.90 ± 3.38 (n = 3), and 98.50 ± 0.42 (n = 3), comparable to High-Performance Liquid Chromatography-Photodiode Array Detector results. This study is novel owing to the peculiar UV-active spectrum fingerprint and the convenient use of hydrophobic film strips that could promote breakthrough innovations and new frontiers for on-site/forensic detection of environmental pollutants.
摘要：
极端微生物曲霉对环境的污染，即，黄曲霉毒素B1在东南亚和撒哈拉以南非洲几乎无法控制，缺乏处理资源和受控存储设施。易感染黄曲霉毒素的饮食主食引起的急性黄曲霉毒素中毒可引起急性肝坏死，急性肝功能衰竭,和死亡。这里,越便宜，更直接,和方便的现场诊断试剂盒是必要的，我们报告了一种基于氟化乙丙橡胶薄膜条的紫外激发光学传感器。适体的分子动力学。AFB1复合物显示AFB1对适体的整体结构稳定性增加，这表明适体设计适合于预期的应用。在各种影响因素下，拟议的无标签策略提供了快速20分钟的现场制造简单性和19天的保质期。一锅孵育提供了催化检测的替代方案，并表现出4倍的可重用性。粗红糖的回收，花生加工,长粒水稻为102.74±0.41(n=3)，86.90±3.38（n=3），98.50±0.42(n=3)，与高效液相色谱-光电二极管阵列检测器结果相当。这项研究是新颖的，因为它具有独特的紫外线活性光谱指纹和疏水性薄膜条的便捷使用，可以促进突破性的创新和环境污染物现场/法医检测的新前沿。
