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Abstract：
Cerium oxide nanoparticles possess unique properties that make them promising candidates in various fields, including cancer treatment. Among the proposed synthesis methods for CNPs, biosynthesis using natural extracts, offers an eco-friendly and convenient approach for producing CNPs, particularly for biomedical applications. In this study, a novel method of biosynthesis using the aqueous extract of Eucalyptus camaldulensis leaves was used to synthesize CNPs. Scanning electron microscopy and Transmission electron microscopy (TEM) techniques revealed that the synthesized CNPs exhibit a flower-like morphology. The particle size of CNPs obtained using Powder X-ray diffraction peaks and TEM as 13.43 and 39.25 nm. Energy-dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy confirmed the effect of biomolecules during the synthesis process and the formation of CNPs. The cytotoxicity of biosynthesized samples was evaluated using the MTT method demonstrating the potential of these samples to inhibit MCF-7 cancerous cells. The viability of the MCF-7 cell line conducted by live/dead imaging assay confirmed the MTT cytotoxicity method and indicated their potential to inhibit cancerous cells. Furthermore, the successful uptake of CNPs by MCF-7 cancer cells, as demonstrated by confocal microscopy, provides evidence that the intracellular pathway contributes to the anticancer activity of the CNPs. In general, results indicate that the biosynthesized CNPs exhibit significant cytotoxicity against the MCF-7 cancerous cell line, attributed to their high surface area.
摘要：
氧化铈纳米颗粒具有独特的性能，使其在各个领域有前途的候选人，包括癌症治疗。在拟议的CNP合成方法中，使用天然提取物进行生物合成，提供了一种环保和方便的方法来生产CNP，特别是生物医学应用。在这项研究中,使用桉树叶的水提取物进行生物合成的新方法用于合成CNP。扫描电子显微镜和透射电子显微镜（TEM）技术表明，合成的CNP表现出花状形态。使用粉末X射线衍射峰和TEM获得的CNP的粒径为13.43和39.25nm。能量色散X射线光谱和傅里叶变换红外光谱证实了生物分子在合成过程和CNP形成过程中的作用。使用MTT方法评估生物合成样品的细胞毒性,证明这些样品抑制MCF-7癌细胞的潜力。通过活/死成像测定进行的MCF-7细胞系的活力证实了MTT细胞毒性方法并表明它们抑制癌细胞的潜力。此外，MCF-7癌细胞成功摄取CNPs，正如共聚焦显微镜所证明的那样，提供了细胞内途径有助于CNP的抗癌活性的证据。总的来说,结果表明，生物合成的CNPs对MCF-7癌细胞系表现出显著的细胞毒性，归因于它们的高表面积。
