Abstract:
DNA condensates, formed by liquid-liquid phase separation (LLPS), emerge as promising soft matter assemblies for creating artificial cells. The advantages of DNA condensates are their molecular permeability through the surface due to their membrane-less structure and their fluidic property. However, they face challenges in the design of their surface, e.g., unintended fusion and less regulation of permeable molecules. Addressing them, we report surface modification of DNA condensates with DNA origami nanoparticles, employing a Pickering-emulsion strategy. We successfully constructed core-shell structures with DNA origami coatings on DNA condensates and further enhanced the condensate stability toward fusion via connecting DNA origamis by responding to DNA input strands. The \'armoring\' prevented the fusion of DNA condensates, enabling the formation of multicellular-like structures of DNA condensates. Moreover, the permeability was altered through the state change from coating to armoring the DNA condensates. The armored DNA condensates have significant potential for constructing artificial cells, offering increased surface stability and selective permeability for small molecules while maintaining compartmentalized space and multicellular organization.
摘要:
DNA凝聚物,通过液-液相分离(LLPS)形成,作为用于创建人造细胞的有前途的软物质组件出现。DNA缩合物的优点是由于它们的无膜结构和它们的流体性质,它们通过表面的分子渗透性。然而,他们在表面设计方面面临挑战,例如,非预期的融合和对可渗透分子的调节较少。解决他们,我们报道了用DNA折纸纳米颗粒对DNA缩合物进行表面修饰,采用皮克林乳液策略。我们成功地在DNA缩合物上构建了具有DNA折纸涂层的核-壳结构,并通过响应DNA输入链,通过连接DNA起源进一步增强了缩合物对融合的稳定性。“装甲”阻止了DNA凝聚物的融合,能够形成DNA缩合物的多细胞样结构。此外,渗透性通过从涂层到铠装DNA冷凝物的状态变化而改变。装甲的DNA缩合物具有构建人造细胞的巨大潜力,为小分子提供增加的表面稳定性和选择性渗透性,同时保持分隔的空间和多细胞组织。