Abstract：
In this work, carboxymethylated curdlan (CMCD) was utilized as a capping and stabilizing agent for the green synthesis of silver nanoparticles. Subsequently, quaternized curdlan (QCD) was introduced as the second capping layer through electrostatic attraction, leading to the preparation of double-capped silver nanoparticles (AgNPs@CQ). The successful synthesis of silver nanoparticles was characterized using UV-vis, FTIR, XRD, TEM, and DLS. AgNPs@CQ were incorporated into gelatin and a AgNPs@CQ/Gel composite hydrogel was obtained. The incorporation of AgNPs@CQ imparts excellent antibacterial properties to the composite hydrogel, thereby enhancing its antimicrobial efficacy. The presence of double-capping layers significantly retards the release rate of silver, contributing to prolonged antimicrobial activity. The MTT and live/dead fluorescence staining results demonstrate that the gelatin hydrogel incorporating double-capped AgNPs exhibits enhanced cell viability compared to the one incorporating single-capped AgNPs. Additionally, the composite hydrogel exhibits remarkable mechanical strength and adhesive performance. The AgNPs@CQ/Gel composite hydrogel demonstrates a cost-effective and facile preparation, showing significant potential in the field of dressings.
摘要：
在这项工作中,羧甲基化Curdlan（CMCD）被用作绿色合成银纳米颗粒的封端剂和稳定剂。随后，通过静电引力引入季铵化的Curdlan（QCD）作为第二覆盖层，导致制备双端银纳米颗粒(AgNPs@CQ)。使用UV-vis表征了银纳米颗粒的成功合成，FTIR,XRD,TEM,和DLS。将AgNPs@CQ掺入明胶中并获得AgNPs@CQ/Gel复合水凝胶。AgNPs@CQ的掺入赋予复合水凝胶优异的抗菌性能，从而增强其抗菌功效。双盖层的存在显著延缓了银的释放速率,有助于延长抗菌活性。MTT和活/死荧光染色结果表明,与掺入单封端的AgNP相比,掺入双封端的AgNP的明胶水凝胶表现出增强的细胞活力。此外,复合水凝胶具有显著的机械强度和粘合性能。AgNPs@CQ/Gel复合水凝胶证明了一种经济有效且易于制备的方法，在敷料领域显示出巨大的潜力。
