RESULTS: In this study, whole genome of this strain was sequenced and analyzed. This paper summarized the genomic characteristics of strain TTMP2 and analyzed genes related to the synthesis of tetramethylpyrazine. Bacillus sp. TTMP2 has a complete metabolic pathway for acetoin and tetramethylpyrazine metabolism. Gene function was analyzed by COG annotation, GO annotation, KEGG annotation and functional annotations for lipoproteins, carbohydrate-active enzymes, and pathogen-host interactions. Phylogenetic analysis indicated that Bacillus velezensis had the high homology with Bacillus sp. TTMP2. Genomes of 16 Bacillus species cover all genes of Bacillus, suggesting that genus Bacillus has an open pan-genome and can survive in diverse environments.
CONCLUSIONS: The analysis of genome sequencing data from Bacillus sp. TTMP2 showed that its metabolic characteristics could be deeply understood, indicating that this bacterium had a particular role in tetramethylpyrazine synthesis.
结果:在这项研究中,对该菌株的全基因组进行测序和分析。本文总结了TTMP2菌株的基因组特征,并分析了与川芎嗪合成相关的基因。Bacillussp.TTMP2具有完整的代谢途径,可用于乙酰丙酮和四甲基吡嗪的代谢。通过COG注释分析基因功能,GO注释,脂蛋白的KEGG注释和功能注释,碳水化合物活性酶,和病原体-宿主相互作用。系统发育分析表明,巴西芽孢杆菌与芽孢杆菌具有很高的同源性。TTMP2.16种芽孢杆菌的基因组涵盖了芽孢杆菌的所有基因,表明芽孢杆菌属具有开放的全基因组,可以在不同的环境中生存。
结论:对来自芽孢杆菌的基因组测序数据的分析。TTMP2表明其代谢特征可被深入理解,表明该细菌在四甲基吡嗪的合成中起着特殊的作用。