Abstract:
Million tons of tires become waste every year, and the so-called End-of-Life Tires (ELTs) are ground into powder (ELT-dp; size < 0.8 mm) and granules (ELT-dg; 0.8 < size < 2.5 mm) for recycling. The aim of this study was to evaluate the sub-lethal effects of three different concentrations (0.1, 1, and 10 mg/L) of aqueous suspensions from ELT-dp and ELT-dg on Danio rerio (zebrafish) larvae exposed from 0 to 120 h post-fertilization (hpf). Chronic effects were assessed through biomarkers, real-time PCR, and proteomics. We observed a significant increase in swimming behavior and heart rate only in specimens exposed to ELT-dp suspensions at 1 and 10 mg/L, respectively. Conversely, the activities of detoxifying enzymes ethoxyresorufin-O-deethylase (EROD) and glutathione-S-transferase (GST) showed significant modulation only in specimens exposed to ELT-dg groups. Although no effects were observed through real-time PCR, proteomics highlighted alterations induced by the three ELT-dp concentrations in over 100 proteins involved in metabolic pathways of aromatic and nitrogen compounds. The results obtained suggest that the toxic mechanism of action (MoA) of ELT suspensions is mainly associated with the induction of effects by released chemicals in water, with a higher toxicity of ELT-dp compared to ELT-dg.
摘要:
每年百万吨轮胎变成废物,将所谓的报废轮胎(ELTs)磨成粉末(ELT-dp;尺寸<0.8mm)和颗粒(ELT-dg;0.8<尺寸<2.5mm)用于回收。这项研究的目的是评估三种不同浓度(0.1、1和10mg/L)的ELT-dp和ELT-dg水性悬浮液对暴露于0至120h受精后(hpf)的Daniorerio(斑马鱼)幼虫的亚致死效应。通过生物标志物评估慢性效应,实时PCR,和蛋白质组学。我们观察到游泳行为和心率的显着增加,仅在暴露于1和10mg/L的ELT-dp悬浮液的标本中,分别。相反,解毒酶乙氧基间苯二酚-O-脱乙基酶(EROD)和谷胱甘肽-S-转移酶(GST)的活性仅在暴露于ELT-dg组的标本中显示出显着的调节。尽管通过实时PCR没有观察到影响,蛋白质组学强调了三种ELT-dp浓度在涉及芳香族化合物和氮化合物代谢途径的100多种蛋白质中引起的变化。获得的结果表明,ELT悬浮液的毒性作用机制(MoA)主要与水中释放的化学物质的诱导作用有关,与ELT-dg相比,ELT-dp的毒性更高。